Anti-RAB7抗体[Rab7-117] (ab50533)

概述

  • 产品名称Anti-RAB7抗体[Rab7-117]
    参阅全部 RAB7 一抗
  • 描述
    小鼠单克隆抗体[Rab7-117] to RAB7
  • 经测试应用适用于: ICC/IF, IP, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Cow, Dog, Human, Pig, Monkey, Zebrafish
    预测可用于: Rabbit, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    EQAFQTIARNALKQE

    , corresponding to amino acids 163-177 of Human RAB7

  • 阳性对照
    • HeLa total cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab50533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent dilution.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.
  • 组织特异性Widely expressed; high expression found in skeletal muscle.
  • 疾病相关Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant.
  • 序列相似性Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位Late endosome. Lysosome. Cytoplasmic vesicle > phagosome. Melanosome. Cytoplasmic vesicle > phagosome membrane. Co-localizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMT2B antibody
    • PRO2706 antibody
    • PSN antibody
    • RAB7, member RAS oncogene family antibody
    • RAB7A antibody
    • RAB7A, member RAS oncogene family antibody
    • RAB7A_HUMAN antibody
    • Ras associated protein RAB7 antibody
    • Ras related protein Rab7 antibody
    • Ras related protein Rab7a antibody
    • Ras-related protein Rab-7a antibody
    see all

Anti-RAB7 antibody [Rab7-117] 图像

  • ICC/IF image of ab50533 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50533, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/2000 dilution + HepG2 whole cell lysate at 1/2000 dilution

    Secondary
    HRP-conjugated donkey anti-mouse IgG polyclonal at 1/1 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Armen Petrosyan

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.

    See Abreview

  • ab50533 staining RAB7 in HepG2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in PBST + 1% donkey serum) for 3 hours at 22°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/2000 dilution + Rat hepatocytes whole cell lysate at 20 µg

    Secondary
    HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Armen Petrosyan

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.

    See Abreview

  • Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/1000 dilution + Lysate prepared from mouse neuroblastoma cells at 10 µg

    Secondary
    HRP-conjugated goat monoclonal to mouse IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa


    Exposure time : 20 seconds

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/500 dilution (for 3 hours at 20°C) + Human brain whole tissue lysate at 15 µg

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 20°C

    See Abreview

Anti-RAB7 antibody [Rab7-117] (ab50533)参考文献

This product has been referenced in:
  • Fields J  et al. HIV-1 Tat alters neuronal autophagy by modulating autophagosome fusion to the lysosome: implications for HIV-associated neurocognitive disorders. J Neurosci 35:1921-38 (2015). IP ; Mouse . Read more (PubMed: 25653352) »
  • Kanagaraj P  et al. Souffle/Spastizin Controls Secretory Vesicle Maturation during Zebrafish Oogenesis. PLoS Genet 10:e1004449 (2014). ICC/IF ; Zebrafish . Read more (PubMed: 24967841) »

See all 23 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse macrophage cells)
Permeabilization No
Specification mouse macrophage cells
Blocking step BSA as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 30 2015

Application Western blot
Sample Human Cell lysate - whole cell (neuronal cell line)
Gel Running Conditions Non-reduced Denaturing (12%)
Loading amount 30 µg
Treatment alpha synuclein
Specification neuronal cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 12 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2 cells)
Permeabilization Yes - 0.2% Triton X-100
Specification HepG2 cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jul 27 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat hepatocytes)
Permeabilization Yes - 0.2% Triton X-100
Specification Rat hepatocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jul 27 2015

Application Western blot
Sample Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Loading amount 25 µg
Specification HepG2 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jul 27 2015

Application Western blot
Sample Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Loading amount 20 µg
Specification Rat hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jul 27 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Human Cell (Ovarian carcinoma (A2780))
Specification Ovarian carcinoma (A2780)
Permeabilization Yes - 0.1% Triton-x100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 18 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (Primary mouse hippocampal neurons)
Specification Primary mouse hippocampal neurons
Permeabilization Yes - 0.25 % TX 100
Fixative Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Dec 09 2013

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Pig Cell lysate - whole cell (Pig retinal pigment epithelium (RPE) cells)
Specification Pig retinal pigment epithelium (RPE) cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Dec 09 2013

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Dog Cell lysate - whole cell (MDCK II cells)
Specification MDCK II cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Dec 09 2013

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