概述

  • 产品名称Anti-RAB7抗体
    参阅全部 RAB7 一抗
  • 描述
    兔多克隆抗体to RAB7
  • 经测试应用适用于: IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Chimpanzee, Monkey, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a portion of the amino acids 90-140 of Human RAB7A

  • 阳性对照
    • Skeletal muscle tissue.

性能

应用

Our Abpromise guarantee covers the use of ab77993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 22781172
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.
  • 组织特异性Widely expressed; high expression found in skeletal muscle.
  • 疾病相关Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant.
  • 序列相似性Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位Late endosome. Lysosome. Cytoplasmic vesicle > phagosome. Melanosome. Cytoplasmic vesicle > phagosome membrane. Co-localizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMT2B antibody
    • PRO2706 antibody
    • PSN antibody
    • RAB7, member RAS oncogene family antibody
    • RAB7A antibody
    • RAB7A, member RAS oncogene family antibody
    • RAB7A_HUMAN antibody
    • Ras associated protein RAB7 antibody
    • Ras related protein Rab7 antibody
    • Ras related protein Rab7a antibody
    • Ras-related protein Rab-7a antibody
    see all

Anti-RAB7 antibody 图像

  • ab77993 at 10µg/ml staining RAB7 in skeletal muscle tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • All lanes : Anti-RAB7 antibody (ab77993) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 34 kDa,49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-RAB7 antibody (ab77993)参考文献

This product has been referenced in:
  • Sathyanesan M  et al. A molecular characterization of the choroid plexus and stress-induced gene regulation. Transl Psychiatry 2:e139 (2012). IHC-Fr ; Rat . Read more (PubMed: 22781172) »
  • Baird NL  et al. Arenavirus infection induces discrete cytosolic structures for RNA replication. J Virol 86:11301-10 (2012). Read more (PubMed: 22875974) »

See all 3 Publications for this product

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Thank you for contacting us. I am sorry to hear that ab77993 is giving no signal on your samples. I would expect the protocol you are using to work well. If you have purchased the antibody in the past six months, I would be happy to offer you a free of...

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Application Immunocytochemistry/ Immunofluorescence
Sample Chicken Cell (chicken embryonic epithelial cell)
Specification chicken embryonic epithelial cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Tween
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
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Ms. Chyong-Yi Wu

Verified customer

提交于 Feb 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"