Anti-Rab5抗体[Rab5-65] (ab50523)

概述

  • 产品名称Anti-Rab5抗体[Rab5-65]
    参阅全部 Rab5 一抗
  • 描述
    小鼠单克隆抗体[Rab5-65] to Rab5
  • 经测试应用适用于: Indirect ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cat, Dog, Human
    预测可用于: Chicken, Cow, Pig, Zebrafish, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    ASRGATRPNGPNT

    conjugated to KLH, corresponding to amino acids 2-14 of Human Rab5

  • 阳性对照
    • Total cell extract of HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab50523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA
ICC/IF
WB
  • 应用说明ICC/IF: Use at an assay dependent dilution.
    I-ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
    Array: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Rab5 antibody [Rab5-65] 图像

    • ICC of Hela cells, using ethanol/methanol fixation (10 min/1 min) at –20 °C. Antibody concentration 4 µg/mL. Anti-Mouse Fab-Cy3, as secondary Ab.


    • Predicted band size : 24 kDa
      Observed band size : 24 kDa

    Anti-Rab5 antibody [Rab5-65] (ab50523)参考文献

    This product has been referenced in:
    • Farg MA  et al. C9ORF72, implicated in amytrophic lateral sclerosis and frontotemporal dementia, regulates endosomal trafficking. Hum Mol Genet N/A:N/A (2014). WB . Read more (PubMed: 24549040) »
    • Harriff MJ  et al. Human lung epithelial cells contain Mycobacterium tuberculosis in a late endosomal vacuole and are efficiently recognized by CD8? T cells. PLoS One 9:e97515 (2014). Read more (PubMed: 24828674) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Pig Cell (Tracheobronchal epithelial cell and Intestinal epi)
    Permeabilization Yes - Saponin 0.2%
    Specification Tracheobronchal epithelial cell and Intestinal epi
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Mr. Sébastien Holbert

    Verified customer

    提交于 Sep 27 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Pancreas)
    Gel Running Conditions Reduced Denaturing (12.5%)
    Loading amount 30 µg
    Specification Pancreas
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 20 2016

    Thank you for your email.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab66746.
    Read More

    Thank you for contacting us.

    Yes, I'd be happy to send you one of the mouse antibodies, but am afraid I cannot make the final decision for you. I'd be able to make a recommendation, though.
    Both antibodies (ab66746,ab55657) have not bee...

    Read More

    Thank you for your reply and the additional information.

    If you see no change in the staining pattern even with more dilute antibody solutions, that seems to point more towards non-specific binding of the antibody. And I am not sure that a d...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HEK-T)
    Specification HEK-T
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    提交于 Apr 30 2012

    Thank you for sharing your results through our Abreview system. I am sorry this antibody did not provide the expected results.

    Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"