Anti-Rab5抗体- Drosophila Early Endosome Marker (ab31261)
Key features and details
- Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Rab5抗体- Drosophila Early Endosome Marker
参阅全部 Rab5 一抗 -
描述
兔多克隆抗体to Rab5 - Drosophila Early Endosome Marker -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Drosophila melanogaster -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31261于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
靶标
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功能
Required for the fusion of plasma membranes and early endosomes. -
序列相似性
Belongs to the small GTPase superfamily. Rab family. -
细胞定位
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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别名
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
图片
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Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml + Schneider L2 whole cell lysate (ab14893) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 135 kDa, 145 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (62)
ab31261 被引用在 62 文献中.
- O'Brien CE et al. The GARP complex prevents sterol accumulation at the trans-Golgi network during dendrite remodeling. J Cell Biol 222:N/A (2023). PubMed: 36239632
- Xu L et al. ER-mitochondrial contact protein Miga regulates autophagy through Atg14 and Uvrag. Cell Rep 41:111583 (2022). PubMed: 36323251
- Wagner K et al. Phospholipase D and retromer promote recycling of TRPL ion channel via the endoplasmic reticulum. Traffic 23:42-62 (2022). PubMed: 34719094
- Ding X et al. Toll-7 promotes tumour growth and invasion in Drosophila. Cell Prolif 55:e13188 (2022). PubMed: 35050535
- Tanasic D et al. Myosin V facilitates polarised E-cadherin secretion. Traffic 23:374-390 (2022). PubMed: 35575181