概述

  • 产品名称
  • 描述
    兔多克隆抗体to Rab5
  • 经测试应用
    适用于: WB, ICC/IF, IHC-P, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Cow, Human, Monkey
    预测可用于: Dog
  • 免疫原

    Synthetic peptide:

    PKNEPQNPGANSARGR

    conjugated to KLH, corresponding to amino acids 182-197 of Human Rab5 (Rab5A)

  • 阳性对照
    • Mouse or Rat Brain Tissue.

性能

应用

Our Abpromise guarantee covers the use of ab13253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.8 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 19348413
IHC-P 1/100.

Use Bouin's fixative to fix tissue.

ICC Use at an assay dependent concentration.

靶标

图片

  • Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of polyclonal rabbit anti-Rab5 (Abcam ab13253, 1/200; Alexa Fluor 488; 1/200; green) and monoclonal mouse anti-tubulin-II (Alexa Fluor 568 1/200; red). Magnification 63x; insert 180x.
  • ab13253 Rabbit polyclonal to Rab5 was used in in fixed HEK cells that had been transfected with Rab5-GFP Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling of rabbit polyclonal Rab5 (ab13253, 1/500; Cy3; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 7µm. Showing good co-localisation.

  • ab13253 at 1/100 dilution staining Rab5 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.

  • Immunofluorescence analysis of HeLa cells, staining Rab5 (green) with ab13253.

    Cells were incubated for 30 min with 0.5 µM biotinylated transportan and fixed with PLP fixative. Cells were permeabilized and blocked with 0.01% saponin and 0.1% BSA for 8 minutes, before incubating with primary antibody. An AlexFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

文献

This product has been referenced in:
  • Altadill T  et al. Enabling Metabolomics Based Biomarker Discovery Studies Using Molecular Phenotyping of Exosome-Like Vesicles. PLoS One 11:e0151339 (2016). WB . Read more (PubMed: 26974972) »
  • Takeda S  et al. Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer's disease brain. Nat Commun 6:8490 (2015). ICC/IF . Read more (PubMed: 26458742) »

See all 21 Publications for this product

客户评价及客户问答

Thank you for contacting us.

The antibody ab13253 is guaranteed for ICC/IF in mouse cells; this means this product comes with fully money back guarantee if used as stated on the datasheet.

We unfortunately do not have any mouse specif...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HeLa)
Loading amount
100000 cells
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Mar 31 2009

Thank you for your patience, and I apologize for the delay in answering your question. We have not personally tested this antibody for IHC/IF but it has been cited in a publication: http://www.jbc.org/cgi/content/full/276/21/18540 As one would ex...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cell (Hippocampal neuron culture)
Specification
Hippocampal neuron culture
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

提交于 Dec 02 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (human Rab5-transfected HEK cells)
Specification
human Rab5-transfected HEK cells
Fixative
Methanol
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

提交于 Dec 02 2005

Thank you for your enquiry and for taking your time to fill in the on-line questionnaire. We are very sorry to hear that you are having problem with this antibody. According to our order record and the database, we have sold several vials of this b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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