概述

  • 产品名称Anti-Rab5抗体
    参阅全部 Rab5 一抗
  • 描述
    兔多克隆抗体to Rab5
  • 经测试应用适用于: WB, ICC/IF, IHC-P, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Cow, Human, Monkey
    预测可用于: Dog
  • 免疫原

    Synthetic peptide:

    PKNEPQNPGANSARGR

    conjugated to KLH, corresponding to amino acids 182-197 of Human Rab5 (Rab5A)

  • 阳性对照
    • Mouse or Rat Brain Tissue.

性能

应用

Our Abpromise guarantee covers the use of ab13253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.8 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 19348413
IHC-P 1/100.

Use Bouin's fixative to fix tissue.

ICC Use at an assay dependent concentration.

靶标

Anti-Rab5 antibody 图像

  • Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of polyclonal rabbit anti-Rab5 (Abcam ab13253, 1/200; Alexa Fluor 488; 1/200; green) and monoclonal mouse anti-tubulin-II (Alexa Fluor 568 1/200; red). Magnification 63x; insert 180x.
  • ab13253 Rabbit polyclonal to Rab5 was used in in fixed HEK cells that had been transfected with Rab5-GFP Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling of rabbit polyclonal Rab5 (ab13253, 1/500; Cy3; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 7µm. Showing good co-localisation.

  • ab13253 at 1/100 dilution staining Rab5 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.

  • Immunofluorescence analysis of HeLa cells, staining Rab5 (green) with ab13253.

    Cells were incubated for 30 min with 0.5 µM biotinylated transportan and fixed with PLP fixative. Cells were permeabilized and blocked with 0.01% saponin and 0.1% BSA for 8 minutes, before incubating with primary antibody. An AlexFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

Anti-Rab5 antibody (ab13253)参考文献

This product has been referenced in:
  • Tome ME  et al. Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels. J Neurochem 134:200-10 (2015). Read more (PubMed: 25832806) »
  • Mottola G  et al. Tropheryma whipplei, the agent of Whipple's disease, affects the early to late phagosome transition and survives in a Rab5- and Rab7-positive compartment. PLoS One 9:e89367 (2014). Read more (PubMed: 24586722) »

See all 16 Publications for this product

Product Wall

Thank you for contacting us.

The antibody ab13253 is guaranteed for ICC/IF in mouse cells; this means this product comes with fully money back guarantee if used as stated on the datasheet.

We unfortunately do not have any mouse specif...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa)
Loading amount 100000 cells
Specification HeLa
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Mar 31 2009

Thank you for your patience, and I apologize for the delay in answering your question. We have not personally tested this antibody for IHC/IF but it has been cited in a publication: http://www.jbc.org/cgi/content/full/276/21/18540 As one would ex...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cell (Hippocampal neuron culture)
Specification Hippocampal neuron culture
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

提交于 Dec 02 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (human Rab5-transfected HEK cells)
Specification human Rab5-transfected HEK cells
Fixative Methanol
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

提交于 Dec 02 2005

Thank you for your enquiry and for taking your time to fill in the on-line questionnaire. We are very sorry to hear that you are having problem with this antibody. According to our order record and the database, we have sold several vials of this b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"