概述

  • 产品名称
    Anti-Rab5抗体[3A4]
    参阅全部 Rab5 一抗
  • 描述
    小鼠单克隆抗体[3A4] to Rab5
  • 经测试应用
    适用于: WB, ELISA, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 1-215 of Human Rab5 purified from E. coli.

  • 阳性对照
    • Extracts of mouse brain.

性能

应用

Our Abpromise guarantee covers the use of ab66746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 2-5µg for 106 cells.

靶标

图片



  • Predicted band size : 24 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Hu brain whole cell lysate (20 µg)
    Lane 4: Ms brain whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.

  • ab66746 staining Rab5 in the U87MG human cell line at a dilution of 1/100. 

    DAPI was used for nuclear staining.

  • Anti-Rab5 antibody [3A4] (ab66746) at 1/500 dilution + extracts of mouse brain at 45 µg

    Secondary
    HRP-conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • Flow cytometry analysis of ab66746 staining Rab5 in HeLa cells. Primary antibody was used at a concentration of 2-5ug per 1 X 106 cells. The secondary antibody used was an Alexa fluor 488® conjugated goat anti-mouse IgG.

文献

This product has been referenced in:
  • Del Cid-Pellitero E  et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. Front Syst Neurosci 11:17 (2017). Read more (PubMed: 28408870) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 09 2016

Thank you for your email.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab66746.
Read More

Thank you for contacting us.

Yes, I'd be happy to send you one of the mouse antibodies, but am afraid I cannot make the final decision for you. I'd be able to make a recommendation, though.
Both antibodies (ab66746,ab55657) have not bee...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册