使用敲除细胞株进行验证RabMAb

Anti-Rab4抗体[EPR3043] - Early Endosome Marker (ab109009)

概述

  • 产品名称Anti-Rab4抗体[EPR3043] - Early Endosome Marker
    参阅全部 Rab4 一抗
  • 描述
    兔单克隆抗体[EPR3043] to Rab4 - Early Endosome Marker
  • 经测试应用适用于: WB, IP, ICC/IFmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Rab4 aa 150-250 (C terminal).

  • 阳性对照
    • MCF7, PC12, Neuro 2a, 293T, SH SY5Y and Human fetal brain lysates; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IP 1/10 - 1/100.
ICC/IF 1/500 - 1/1000.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    Anti-Rab4 antibody [EPR3043] - Early Endosome Marker 图像



    • Predicted band size : 24 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human fetal brain lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109009 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109009 was shown to specifically react with Rab4 when Rab4 knockout samples were used. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab109009 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : Neuro 2a cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : SH SY5Y cell lysate
      Lane 6 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 24 kDa
    • ab109009 at 1/500 dilution staining Rab4 in HeLa by Immunofluorescence.

    Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)参考文献

    ab109009 has not yet been referenced specifically in any publications.

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