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Our Abpromise guarantee covers the use of ab55656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human fetal brain lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab55656 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab55656 was shown to specifically react with Rab4 when Rab4 knockout samples were used. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab55656 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.