概述

  • 产品名称Anti-Rab4抗体
    参阅全部 Rab4 一抗
  • 描述
    小鼠单克隆抗体to Rab4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-219 of Human Rab4

性能

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应用

Our Abpromise guarantee covers the use of ab55656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.

靶标

  • 功能Protein transport. Probably involved in vesicular traffic.
  • 序列相似性Belongs to the small GTPase superfamily. Rab family.
  • 翻译后修饰Phosphorylated by CDK1 kinase during mitosis.
  • 细胞定位Membrane. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HRES 1 / RAB4 antibody
    • Oncogene RAB4 antibody
    • Rab 4 antibody
    • RAB 4A antibody
    • RAB4 member RAS oncogene family antibody
    • Rab4a antibody
    • RAB4A member RAS oncogene family antibody
    • RAB4A_HUMAN antibody
    • Ras related protein Rab 4A antibody
    • Ras related protein Rab4A antibody
    • Ras-related protein Rab-4A antibody
    see all

Anti-Rab4 antibody 图像



  • Predicted band size : 24 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Human fetal brain lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab55656 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab55656 was shown to specifically react with Rab4 when Rab4 knockout samples were used. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab55656 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.



  • Predicted band size : 24 kDa
    Rab4 antibody (ab55656) at 1ug/lane + HeLa cell lysate at 25ug/lane.

Anti-Rab4 antibody (ab55656)参考文献

This product has been referenced in:
  • Potokar M  et al. Rab4 and Rab5 GTPase are required for directional mobility of endocytic vesicles in astrocytes. Glia 60:594-604 (2012). Read more (PubMed: 22279005) »

See 1 Publication for this product

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