概述

  • 产品名称
  • 描述
    兔多克隆抗体to RAB3IP
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (PDLLGVYESG TQEQTTSPSV IYRPHPSALS SVPIQANALD VSELPTQPVY) of Human RAB3IP (NP_783322).

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab90732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.
  • 组织特异性
    Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.
  • 序列相似性
    Belongs to the SEC2 family.
  • 细胞定位
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gtpat12 antibody
    • Rab-3A-interacting protein antibody
    • RAB3A interacting protein antibody
    • RAB3A interacting protein (rabin3) antibody
    • RAB3A interacting protein antibody
    • RAB3A-interacting protein antibody
    • RAB3I_HUMAN antibody
    • Rab3ip antibody
    • Rabin 3 antibody
    • Rabin-3 antibody
    • RABIN3 antibody
    • RABIN3, rat, homolog of antibody
    • Rabin8 antibody
    • SSX2 interacting protein antibody
    • SSX2 interacting protein antibody
    • SSX2-interacting protein antibody
    see all

图片

  • Primary Antibody Dilution : 1:200. Secondary Antibody : Goat anti-rabbit Alexafluor 568. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : RAB3IP: Red CtBp: Green DAPI: Blue. Gene Name : RAB3IP.
  • Primary Antibody Dilution : 1:200. Secondary Antibody : Goat anti-rabbit Alexafluor 568. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : RAB3IP: Red CtBp: Green DAPI: Blue. Gene Name : RAB3IP.
  • Primary Antibody Dilution : 1:200. Secondary Antibody : Goat anti-rabbit Alexafluor 568. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : RAB3IP: Red CtBp: Green DAPI: Blue. Gene Name : RAB3IP.
  • Anti-RAB3IP antibody (ab90732) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Gel concentration: 12%

文献

This product has been referenced in:
  • Liu Y  et al. KRASG12 mutant induces the release of the WSTF/NRG3 complex, and contributes to an oncogenic paracrine signaling pathway. Oncotarget 7:53153-53164 (2016). Read more (PubMed: 27449290) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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