概述

  • 产品名称Anti-Rab3D抗体
    参阅全部 Rab3D 一抗
  • 描述
    兔多克隆抗体to Rab3D
  • 特异性Detects recombinant mouse rab 3D. This antibody does not detect rab 3A, 3B, or 3C.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Rat Rab3D aa 1-18.
    Sequence:

    MASASEPPASPRDAADQN


    (Peptide available as ab4953)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • Primary antibody说明Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab3337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 24 kDa).Can be blocked with Rab3D peptide (ab4953).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Protein transport. Probably involved in regulated exocytosis.
  • 组织特异性Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated.
  • 序列相似性Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D2-2 antibody
    • Glioblastoma overexpressed antibody
    • GOV antibody
    • RAB 16 antibody
    • RAB16 antibody
    • Rab3d antibody
    • Rab3D upregulated with myeloid differentiation antibody
    • RAB3D, member RAS oncogene family antibody
    • RAB3D_HUMAN antibody
    • RAD3D antibody
    • Ras-related protein Rab-3D antibody
    see all

Anti-Rab3D antibody (ab3337)参考文献

This product has been referenced in:
  • Liew CW  et al. The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells. J Clin Invest 120:2876-88 (2010). WB ; Mouse . Read more (PubMed: 20592469) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Loading amount 20 µg
Specification Rat hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jun 18 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2 cells)
Permeabilization Yes - 0.2% Triton X-100
Specification HepG2 cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 27 2015

Application Western blot
Sample Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Loading amount 20 µg
Specification HepG2 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 30 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat hepatocytes)
Permeabilization Yes - 0.2% Triton X-100
Specification Rat hepatocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 13 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (pancreatic beta-cells)
Loading amount 20 µg
Specification pancreatic beta-cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Oct 12 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"