概述

  • 产品名称
  • 描述
    兔多克隆抗体to Rab3D
  • 特异性
    Detects recombinant mouse rab 3D. This antibody does not detect rab 3A, 3B, or 3C.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Rat Rab3D aa 1-18.
    Sequence:

    MASASEPPASPRDAADQN


    (Peptide available as ab4953)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • Primary antibody说明
    Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 24 kDa).Can be blocked with Rab3D peptide (ab4953).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Protein transport. Probably involved in regulated exocytosis.
  • 组织特异性
    Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated.
  • 序列相似性
    Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D2-2 antibody
    • Glioblastoma overexpressed antibody
    • GOV antibody
    • RAB 16 antibody
    • RAB16 antibody
    • Rab3d antibody
    • Rab3D upregulated with myeloid differentiation antibody
    • RAB3D, member RAS oncogene family antibody
    • RAB3D_HUMAN antibody
    • RAD3D antibody
    • Ras-related protein Rab-3D antibody
    see all

Anti-Rab3D antibody (ab3337)参考文献

This product has been referenced in:
  • Liew CW  et al. The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells. J Clin Invest 120:2876-88 (2010). WB ; Mouse . Read more (PubMed: 20592469) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Loading amount
20 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jun 18 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 27 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Loading amount
20 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 30 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 13 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (pancreatic beta-cells)
Loading amount
20 µg
Specification
pancreatic beta-cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Oct 12 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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