概述

  • 产品名称
  • 描述
    兔多克隆抗体to Rab3C
  • 经测试应用
    适用于: WB, IP, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Rat Rab3C aa 9-26.
    Sequence:

    MASAQDARFGQKDSSDQN


    (Peptide available as ab4952)

  • 阳性对照
    • AtT20 cells.

性能

应用

Our Abpromise guarantee covers the use of ab3336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Can be blocked with Rab3C peptide (ab4952).
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19786578

靶标

Anti-Rab3C antibody 图像

  • Western blot of Rab3C on AtT20 cell lysate using ab3336.

Anti-Rab3C antibody (ab3336)参考文献

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal Cord)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Spinal Cord
Blocking step
1%BSA+10%FBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 Oct 03 2016

Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 08 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 08 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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