概述

  • 产品名称
  • 描述
    兔多克隆抗体to Rab3A
  • 经测试应用
    适用于: ICC/IF, IP, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Dog, Human
    预测可用于: Cow, Pig, Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Rab3A aa 1-18.
    Sequence:

    MASATDSRYGQKESSDQN


    (Peptide available as ab4951)

  • 阳性对照
    • Hepa1 cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • Primary antibody说明
    Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals and the best studied is rab 3A. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. Abnormal accumulation of rab 3A in the cytoplasm of Purkinje cells has been reported in the prion protein related Creutzfeldt-Jakob disease.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Can be blocked with Rab3A peptide (ab4951).
IHC-Fr 1/300.

靶标

图片

  • Western blot detection of Rab3A on Hepa1 cell lysate using ab3335.

  • ab3335 staining hamster CHO-K1 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour at 37°C.  The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

文献

This product has been referenced in:
  • Spencer B  et al. Reducing Endogenous a-Synuclein Mitigates the Degeneration of Selective Neuronal Populations in an Alzheimer's Disease Transgenic Mouse Model. J Neurosci 36:7971-84 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 27466341) »
  • Mohankumar KM  et al. An in vivo screen identifies ependymoma oncogenes and tumor-suppressor genes. Nat Genet 47:878-87 (2015). WB . Read more (PubMed: 26075792) »

See all 7 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 05 2015

Application
Western blot
Sample
Human Tissue lysate - whole (brain tissue)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 05 2015

Application
Immunoprecipitation
Immuno-precipitation step
Other - magnetic Dynabeads
Sample
Mouse Tissue lysate - whole (brain)
Specification
brain
Total protein in input
500 µg
Username

Abcam user community

Verified customer

提交于 Feb 10 2015

Application
Western blot
Loading amount
2 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification
hippocampus tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Abcam user community

Verified customer

提交于 Jun 18 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Human Tissue lysate - whole (BRAIN)
Specification
BRAIN
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3)
Loading amount
30 µg
Specification
NIH3T3
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Loading amount
30 µg
Specification
Hippocampus
Gel Running Conditions
Reduced Denaturing (12% TGS)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO-K1)
Specification
CHO-K1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Apr 11 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Rat brain)
Specification
Rat brain
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

提交于 Aug 28 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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