概述

  • 产品名称Anti-Rab3A抗体
    参阅全部 Rab3A 一抗
  • 描述
    兔多克隆抗体to Rab3A
  • 经测试应用适用于: ICC/IF, IP, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Dog, Human
    预测可用于: Cow, Pig, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Rab3A aa 1-18.
    Sequence:

    MASATDSRYGQKESSDQN


    (Peptide available as ab4951)

  • 阳性对照
    • Hepa1 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • Primary antibody说明Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals and the best studied is rab 3A. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. Abnormal accumulation of rab 3A in the cytoplasm of Purkinje cells has been reported in the prion protein related Creutzfeldt-Jakob disease.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab3335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Can be blocked with Rab3A peptide (ab4951).
IHC-Fr 1/300.

靶标

Anti-Rab3A antibody 图像

  • Western blot detection of Rab3A on Hepa1 cell lysate using ab3335.

  • ab3335 staining hamster CHO-K1 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour at 37°C.  The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

Anti-Rab3A antibody (ab3335)参考文献

This product has been referenced in:
  • Annangudi SP  et al. Neuropeptide Release is Impaired in a Mouse Model of Fragile X Mental Retardation Syndrome. ACS Chem Neurosci 1:306-314 (2010). WB ; Human . Read more (PubMed: 20495672) »
  • Rastaldi MP  et al. Glomerular podocytes contain neuron-like functional synaptic vesicles. FASEB J 20:976-8 (2006). Read more (PubMed: 16585060) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jun 05 2015

Application Western blot
Sample Human Tissue lysate - whole (brain tissue)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jun 05 2015

Application Immunoprecipitation
Immuno-precipitation step Other - magnetic Dynabeads
Sample Mouse Tissue lysate - whole (brain)
Specification brain
Total protein in input 500 µg
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提交于 Feb 10 2015

Application Western blot
Loading amount 2 µg
Gel Running Conditions Non-reduced Denaturing
Sample Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification hippocampus tissue lysate
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
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提交于 Jun 18 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing
Sample Human Tissue lysate - whole (BRAIN)
Specification BRAIN
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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提交于 Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH3T3)
Loading amount 30 µg
Specification NIH3T3
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 May 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hippocampus)
Loading amount 30 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (12% TGS)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
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提交于 Sep 19 2008

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Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (CHO-K1)
Specification CHO-K1
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
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提交于 Apr 11 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Rat brain)
Specification Rat brain
Fixative Paraformaldehyde
Permeabilization No
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Dr. Sophie Pezet

Verified customer

提交于 Aug 28 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"