The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能May be involved in melanosomal transport and docking. Involved in the proper sorting of TYRP1.
组织特异性Expressed in melanocytes.
序列相似性Belongs to the small GTPase superfamily. Rab family.
翻译后修饰Although at least one in vitro system can process and methylate the prenylated C-terminal, in an in vitro system that normally express Rab-38 and in vivo the prenylated C-terminal is not proteolytically processed and not methylated.
Predicted band size : 24 kDa Western blot analysis of HeLa (lane 1) and NIHT3 (lane 2) cell lysates labeling RAB38 with ab86542 at 1:1000. A HRP-conjugated Goat anti-rabbit (1:1000) was used as the secondary antibody.
Western blot - RAB38 antibody (ab86542)
Anti-RAB38 antibody (ab86542) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution