Anti-RAB30抗体(ab76622)

概述

  • 产品名称Anti-RAB30抗体
    参阅全部 RAB30 一抗
  • 描述
    小鼠单克隆抗体to RAB30
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant full length human RAB30 protein (AAH14213) with tag.

  • 阳性对照
    • Recombinant tagged full length human RAB30.

性能

应用

Our Abpromise guarantee covers the use of ab76622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 23 kDa.

    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 序列相似性Belongs to the small GTPase superfamily. Rab family.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5033421K01Rik antibody
      • AI323892 antibody
      • OTTMUSP00000025677 antibody
      • OTTMUSP00000025678 antibody
      • RAB 30 antibody
      • Rab30 antibody
      • RAB30 member RAS oncogene family antibody
      • RAB30_HUMAN antibody
      • Ras related protein Rab 30 antibody
      • Ras-associated protein Rab30 antibody
      • Ras-related protein Rab-30 antibody
      • Rsb30 antibody
      see all

    Anti-RAB30 antibody 图像

    • Anti-RAB30 antibody (ab76622) at 1 µg/ml + Recombinant tagged full length human RAB30 at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 23 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    Anti-RAB30 antibody (ab76622)参考文献

    ab76622 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (A549 lung epithelial)
    Specification A549 lung epithelial
    Fixative 4% formaldehyde
    Permeabilization Yes - 0.2% triton x100 in PBS
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 16 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A549 lung epithelial)
    Loading amount 20000 cells
    Specification A549 lung epithelial
    Treatment siRNA treated
    Gel Running Conditions Reduced Denaturing (15%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"