概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to RAB27A
  • 经测试应用
    适用于: WB, IHC-P, IP, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit
  • 免疫原

    Recombinant fragment: YCENPDIVLC GNKSDLEDQR VVKEEEAIAL AEKYGIPYFE TSAANGTNIS QAIEMLLDLI MKRMERCVDK SWIPEGVVRS NGHASTDQLS EEKEKGACGC , corresponding to amino acids 122-222 of Human RAB27A

性能

应用

Our Abpromise guarantee covers the use of ab55667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 3 µg/ml.
IP Use at an assay dependent concentration. See Abreview.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21832089
ICC/IF 1/200.

靶标

  • 功能
    Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
  • 组织特异性
    Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
  • 疾病相关
    Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2) [MIM:607624]. Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome.
  • 序列相似性
    Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位
    Membrane. Melanosome. Late endosome. Lysosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GS2 antibody
    • GTP-binding protein Ram antibody
    • HsT18676 antibody
    • MGC117246 antibody
    • Mutant Ras related protein Rab-27A antibody
    • Rab-27 antibody
    • RAB-27A antibody
    • RAB27 antibody
    • RAB27A antibody
    • RAB27A member RAS oncogene family antibody
    • RAM antibody
    • Ras-related protein Rab-27A antibody
    • Ras-related protein Rab27A antibody
    • RB27A_HUMAN antibody
    see all

Anti-RAB27A antibody 图像



  • Predicted band size : 25 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: RAB27A knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab55667 observed at 27 kDa. Red - loading control, ab181602, observed at 37 kDa.

  • Anti-RAB27A antibody (ab55667) + HL-60 cell lysate

    Predicted band size : 25 kDa
  • Immunocytochemistry/ Immunofluorescence  analysis of SK-MEL-28 cells labeling RAB27A with ab55667 at 1/200 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton-X in PBS. A polyclonal goat anti-mouse IgG Alexa Fluor® 488 secondary antibody was used at 1/1000 dilution.

    See Abreview

  • All lanes : Anti-RAB27A antibody (ab55667)

    Lane 1 : RAB27A over-expressed 293 cell line, cotransfected with RAB27A Validated Chimera RNAi
    Lane 2 : Non-transfected control


    Predicted band size : 25 kDa

    GAPDH (36.1 kDa) used as specificity and loading control.

  • All lanes : Anti-RAB27A antibody (ab55667)

    Lane 1 : RAB27A transfected lysate(24.9 KDa)
    Lane 2 : Non-transfected lysate


    Predicted band size : 25 kDa
  • RAB27A antibody (ab55667) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma. Heat induced antigen retrieval with Citrate buffer pH 6 has been performed prior to immunostaining.
  • ab55667 staining cow Retinal Pigment Epithelium (RPE) cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 20 minutes at 25°C.  The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® 546 conjugated goat anti-mouse antibody was used as the secondary.  Rab27a, which localizes to the apical site of the RPE cells, is labeled with red fluorescence. Nuclei were counterstained with DAPI (blue).

    See Abreview

  • All lanes : Anti-RAB27A antibody (ab55667) at 1/200 dilution

    Lane 1 : whole cell lysate of HEK 293 transfected with empty pCDNA3 vector
    Lane 2 : whole cell lysate of HEK 293 cells expressing Rab27a-GFP

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 minutes

    This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

    Rab27a (25kDa)+GFP(27kDa)=52kDa

    The blot was blocked with 5% milk for 30 minutes at 25°C and incubated with the primary antibody for 12 hours at 4°C.

    See Abreview

  • ab55667 (undiluted) was incubated with whole cell lysate of HEK 293 cells transfected with Rab27a-GFP and a Protein G matrix for 12 hours at 4°C to achieve immunoprecipitation.  20µg of lysate were present in the input.

    ab55667 (diluted 1/200) was also used in the western blotting step.

    Lane 1. lysate of HEK 293 cells expressing Rab27a-GFP
    Lane 2. IP with ab55667
    Lane 3. Not bound fraction

    See Abreview

Anti-RAB27A antibody (ab55667)参考文献

This product has been referenced in:
  • Jiang C  et al. GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary. PLoS Genet 13:e1006535 (2017). Read more (PubMed: 28072828) »
  • Kudo M  et al. Bifunctional effects of O-methylated flavones from Scutellaria baicalensis Georgi on melanocytes: Inhibition of melanin production and intracellular melanosome transport. PLoS One 12:e0171513 (2017). Read more (PubMed: 28182699) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Dermal Microvascular Endothelial Cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
Human Dermal Microvascular Endothelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 23 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (intestinum)
Specification
intestinum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Blocking Solution Zytomed as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Specification
kidney
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SK-MEL-28)
Specification
SK-MEL-28
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X in PBS
Username

Abcam user community

Verified customer

提交于 Oct 18 2012

Selon nos informations vous avez connu quelques difficultés avec ab55667 et avez contacté notre service scientifique (en février).

Nous vous avons donné quelques suggestions pour améliorer le protocole. J...

Read More

Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée d'apprendre que vous avez des difficultés à obtenir de bons résultats avec ab55667.

Merci d'avoir patienté pendant que je discutais avec l...

Read More

Merci pour votre appel. Je suis désolée d'apprendre que ab55667 ne vous donne pas de bons résultats en immunohistochimie.

Veuillez trouver ci-joint un questionnaire, que nous avons discuté, qui nous permet de regrouper le plus d'informations...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with hRab27a-GFP)
Total protein in input
20 µg
Specification
HEK 293 cells transfected with hRab27a-GFP
Immuno-precipitation step
Protein G
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Feb 29 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293 cells transfected with human Rab27a-GFP)
Loading amount
20 µg
Specification
HEK-293 cells transfected with human Rab27a-GFP
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Feb 29 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (Retinal Pigment Epithelium cells)
Specification
Retinal Pigment Epithelium cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5%TX-100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Feb 21 2008

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册