Anti-Rab25抗体(ab45855)

概述

  • 产品名称Anti-Rab25抗体
    参阅全部 Rab25 一抗
  • 描述
    兔多克隆抗体to Rab25
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Rabbit, Horse, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab25.

    (Peptide available as ab69566.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Whole Cell lysates: MDA-MB-361, MCF7, T47D, HeLa, HepG2, Y79, SW480 and Jurkat This antibody also gave a positive signal in the following Tissue lysates: Human Breast Tumour and Rat Thymus

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab45855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 23 kDa).

靶标

  • 功能Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis.
  • 组织特异性Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue.
  • 序列相似性Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位Cell membrane. Cell projection, pseudopodium membrane. Cytoplasmic vesicle. Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CATX 8 antibody
    • CATX-8 antibody
    • CATX8 antibody
    • RAB11C antibody
    • RAB25 antibody
    • RAB25 member RAS oncogene family antibody
    • RAB25_HUMAN antibody
    • Ras related protein Rab 25 antibody
    • Ras-related protein Rab-25 antibody
    • RP11-336K24.10-001 antibody
    see all

Anti-Rab25 antibody 图像

  • All lanes : Anti-Rab25 antibody (ab45855) at 1 µg/ml

    Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : T47D (Human ductal breast epithelial tumor cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 16 kDa,45 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Rab25 antibody (ab45855) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 4 : SW480 whole cell lysate (ab3957)
    Lane 5 : Rat Thymus Tissue Lysate
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 7 : Human breast tumor tissue lysate - total protein (ab30091)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab45855 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45855, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa and MCF7 cells at 5µg/ml.

Anti-Rab25 antibody (ab45855)参考文献

ab45855 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. I have talked to the lab to see if they have any more information about the unspecific proteins that ab45855 recognizes. Unfortunately they have not been able to determine the identification of these bands. They did inform me...

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I am sorry that you are experiencing issues with this antibody. This particular antibody does recognize unspecified bands at 16kDa and 45kDa, and we are still trying to identify what those bands are, but it should also recognize a band at 32kDa, which ...

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