• 产品名称Anti-RAB21抗体
    参阅全部 RAB21 一抗
  • 描述
    兔多克隆抗体to RAB21
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human RAB21.

    (Peptide available as ab89377.)

  • 阳性对照
    • This antibody gave a positive signal in Human liver tissue lysate and in the following whole cell lysates: HepG2; HeLa; A431; Jurkat.



Our Abpromise guarantee covers the use of ab88762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis.
    • 组织特异性Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression.
    • 序列相似性Belongs to the small GTPase superfamily. Rab family.
    • 细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane. Early endosome membrane. Cytoplasmic vesicle membrane. Cleavage furrow. In nonpolarized epithelial Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the apical cytoplasm. During mitosis, in mid-telophase, localized in the ingressing cleavage furrow. In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base of lamellipodia formed by the separating daughter cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • KIAA0118 antibody
      • RAB 21 antibody
      • RAB21 antibody
      • RAB21, member RAS oncogene family antibody
      • RAB21_HUMAN antibody
      • Ras-related protein Rab-21 antibody
      see all

    Anti-RAB21 antibody 图像

    • All lanes : Anti-RAB21 antibody (ab88762) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 5 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 24 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 12.5 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes

    Anti-RAB21 antibody (ab88762)参考文献

    ab88762 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HAEC-human arterial endothelial cells)
    Loading amount 30 µg
    Specification HAEC-human arterial endothelial cells
    Gel Running Conditions Reduced Denaturing (4-15% gradient)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 45°C

    Mrs. Elena Astanina

    Verified customer

    提交于 May 12 2011