概述

  • 产品名称Anti-RAB1B抗体
    参阅全部 RAB1B 一抗
  • 描述
    小鼠单克隆抗体to RAB1B
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 91 - 202 of human RAB1B protein (NP_112243) with a tag

  • 阳性对照
    • A-431 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab76721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
    • 序列相似性Belongs to the small GTPase superfamily. Rab family.
    • 翻译后修饰Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
      AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active.
    • 细胞定位Membrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • RAB1B antibody
      • RAB1B member RAS oncogene family antibody
      • RAB1B_HUMAN antibody
      • Ras related protein Rab1B antibody
      • Ras-related protein Rab-1B antibody
      • Small GTP binding protein antibody
      see all

    Anti-RAB1B antibody 图像

    • Anti-RAB1B antibody (ab76721) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 22 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    • Anti-RAB1B antibody (ab76721) at 5 µg/ml + A431 cell lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 22 kDa
      Observed band size : 22 kDa

    Anti-RAB1B antibody (ab76721)参考文献

    ab76721 has not yet been referenced specifically in any publications.

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