- 数据表
- 具体的参考文献 (3)
- 实验方案
概述
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产品名称Anti-RAB14抗体
参阅全部 RAB14 一抗 -
描述兔多克隆抗体to RAB14
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经测试应用适用于: ICC/IF, ELISA, IHC-P, WBmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Pig, Zebrafish
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免疫原
Synthetic peptide derived from a region between amino acids 165-214 of human RAB14. (Note: the exact amino acid sequence is proprietary).
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阳性对照
- Raji cell lysate, Human kidney tissue
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
纯度IgG fraction
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纯化说明Purified by peptide affinity chromatography method.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab28639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| ELISA | 1/12500. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.5 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
靶标
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功能May be involved in vesicular trafficking and neurotransmitter release.
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序列相似性Belongs to the small GTPase superfamily. Rab family.
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细胞定位Cell membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 51552 Human
- Entrez Gene: 68365 Mouse
- Entrez Gene: 595112 Pig
- Entrez Gene: 94197 Rat
- Entrez Gene: 373104 Zebrafish
- Omim: 612673 Human
- SwissProt: P61106 Human
- SwissProt: Q91V41 Mouse
see all -
别名
- bA165P4.3 antibody
- F protein binding protein 1 antibody
- FBP antibody
see all
Anti-RAB14 antibody 图像
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Western blot - RAB14 antibody (ab28639)Anti-RAB14 antibody (ab28639) at 0.5 µg/ml + Raji cell lysate 10ug.
Secondary
HRP conjugated anti-Rabbit IgG
Predicted band size : 24 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB14 antibody (ab28639)Human kidney tissue stained with ab28639 at X400 magnigication. The arrows point to epithelial cells of the renal tubule [positive label]. -
Immunocytochemistry/ Immunofluorescence - Anti-RAB14 antibody (ab28639)ICC/IF image of ab28639 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28639, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-RAB14 antibody (ab28639)All lanes : Anti-RAB14 antibody (ab28639) at 2 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with blocking peptide at 2
Lysates/proteins at 2 µg/ml per lane.
Predicted band size : 24 kDa
实验方案
Anti-RAB14 antibody (ab28639)参考文献
This product has been referenced in:
- Reed SE et al. A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes. J Cell Sci 126:1931-41 (2013). Mouse . Read more (PubMed: 23444368) »
- Mukhopadhyay A et al. Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early endocytic vesicles. J Cell Sci 124:765-75 (2011). Read more (PubMed: 21303926) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"