antigen sequence: ITKKETFDDL PKWMKMIDKY ASEDAELLLV GNKLDCETDR EITRQQGEKF AQQITGMRFC EASA, corresponding to internal sequence amino acids 124-187 of Human RAB12.
- Human cerebral cortex tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the small GTPase superfamily. Rab family.
Golgi apparatus membrane.
Information by UniProt
- Putative Ras related protein Rab 12 antibody
- RAB12 antibody
- RAB12, member RAS oncogene family antibody
Immunocytochemistry/ Immunofluorescence - Anti-RAB12 antibody (ab122666)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122666 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB12 antibody (ab122666)
ab122666 at 1/50 dilution staining RAB12 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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