Full length protein, corresponding to amino acids 1-652 of Human RAB11FIP5
- RAB11FIP5 transfected 293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 70 kDa).
Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane.
Detected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas.
Contains 1 C2 domain.
Contains 1 FIP-RBD domain.
Binds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg(2+) rather than Ca(2+).
Phosphorylated on serine and threonine residues.
Cytoplasm. Recycling endosome membrane. Mitochondrion membrane.
Information by UniProt
- GAF 1 antibody
- Gaf-1 antibody
- GAF1 antibody
Western blot - RAB11FIP5 antibody (ab68947)
All lanes :
Anti-RAB11FIP5 antibody (ab68947) at 1/500 dilutionLane 1 :
RAB11FIP5 transfected 293T cell lysateLane 2 :
Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.SecondaryAll lanes :
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionPredicted band size:
70 kDaObserved band size:
77 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
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