ab74446 detects endogenous levels of total RAB11FIP4 protein.
Synthetic peptide derived from an internal region of human RAB11FIP4.
HepG2, 293, COLO, HUVEC and HT29 cell extracts.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
ab74446 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP4 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]). The RAB11FIP4 effector protein acts in endosomal trafficking.
Recycling endosome membrane; Peripheral membrane protein. Note: Colocalizes with RAB11A in the endocytic recycling compartment (ERC).
Rab11 family interacting protein 4 antibody
RAB11 FIP4 antibody
Rab11 FIP4 like antibody
Western blot - RAB11FIP4 antibody (ab74446)
All lanes : Anti-RAB11FIP4 antibody (ab74446) at 1/500 dilution Lane 1 : HepG2 cell extracts Lane 2 : 293 cell extracts Lane 3 : COLO cell extracts Lane 4 : HUVEC cell extracts Lane 5 : HT29 cell extracts Lane 6 : 293 cell extracts with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 72 kDa Observed band size : 72 kDa Additional bands at : 20 kDa,40 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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