概述

  • 产品名称
  • 描述
    兔多克隆抗体to Rab11
  • 经测试应用
    适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Dog, Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Non human primates
  • 免疫原

    Synthetic peptide corresponding to Human Rab11 aa 1-17.
    Sequence:

    MGTRDDEYDYLFKVVLIC


    (Peptide available as ab41779)

  • 阳性对照
    • HeLa, PC3 or CV1 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab3612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 18384686
WB Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Rab11 peptide (ab41779). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.
IP Use at an assay dependent concentration. PubMed: 17686995

靶标

图片

  • Immunofluorescence analysis of HeLa cells, staining Rab11 (red) with ab3612.
    Cells were fixed with paraformaldehyde for 10 minutes, blocked in PBS with 5% normal goat serum and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/200). An AlexaFluor®594-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.

  • ab3612 staining HEK 293 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.012% saponin prior to blocking in 1% BSA for 30 minutes at 37°C.  The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 37°C. A biotinylated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.

    This image depicts ab3612 recognizing endogenous Rab11 (red). Rab11 was used as a recycling endosome marker.

    See Abreview



  • Predicted band size : 24 kDa

    ab3612 used on PC3 cell extract.

  • ab3612 staining Rab11 in Canine kidney cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, then incubated with ab3612 at a 1/200 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.

    See Abreview

文献

This product has been referenced in:
  • Del Cid-Pellitero E  et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. Front Syst Neurosci 11:17 (2017). Read more (PubMed: 28408870) »
  • Kim SW  et al. Covalent, Non-Covalent, Encapsulated Nanodrug Regulate the Fate of Intra- and Extracellular Trafficking: Impact on Cancer and Normal Cells. Sci Rep 7:6454 (2017). Read more (PubMed: 28743942) »

See all 12 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hela)
Specification
hela
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. customer customer

Verified customer

提交于 May 09 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pacific oyster Cell lysate - whole cell (Gills proteome)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
25 µg
Specification
Gills proteome
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 28 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (Baby hamster kidney fibroblast cells (BHK-21))
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
10 µg
Specification
Baby hamster kidney fibroblast cells (BHK-21)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 02 2016

Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (Follicular epithelium)
Specification
Follicular epithelium
Username

Mr. Veit Riechmann

Verified customer

提交于 May 14 2015

Thank you for contacting us.

This antibody detects both RAB11A and Rab11B.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply. I would recommend keeping the blocking to one hour and incubating the primary antibody overnight at 4 degrees. I would suggest doing this at 1/300 for ab74906 and at 1/1000 for ab3612. Please let me know if this does not impro...

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Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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Thank you for the further information. It is difficult to select alternatives that are the most 'specific' as we have not tested these antibodies side by side in the same sample application or species. Reviewing the datasheets I think that ab74906 a...

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Thank you for contacting us. Yes, a suggested starting dilution for the primary antibody would be 1/200. And I would suggest keeping the secondary antibody dilution constant while titrating the primary antibody. Once you know the optimal dilution fo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Kidney (ATCC CCL-34))
Specification
Kidney (ATCC CCL-34)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% saponin
Username

Abcam user community

Verified customer

提交于 Oct 07 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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