Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Fruit fly (Drosophila melanogaster) R2D2.
(Peptide available as
This antibody gave a positive signal in Drosophila lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme consists of DCR-2 and R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4.
CG7138 PA antibody
Western blot - Anti-R2D2 antibody (ab14750)
All lanes :
Anti-R2D2 antibody (ab14750) at 1 µg/ml
Lane 1 :
Lane 2 :
Drosophila lysate with Human R2D2 peptide (
) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size:
Observed band size:
37 kDa (
why is the actual band size different from the predicted?
Additional bands at:
17 kDa (possible cross reactivity), 65 kDa (possible cross reactivity)
ab14750 specifically recognises a band at 37kDa corresponding to R2D2, which is blocked using the immunising peptide (
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