概述

  • 产品名称(R)-(-)-Rolipram
  • 描述
    Selective PDE4 inhibitor
  • 生物学描述Selective PDE4 inhibitor (IC50 = 0.22 μM). More active enantiomer: 2-10-fold more potent that the (S)-(+)-enantiomer (ab120030).
  • 纯度> 98%

性能

  • 化学名称(4R)-4-[3-(Cyclopentyloxy)-4-methoxyphenyl]pyrrolidin-2-one
  • 分子量275.35
  • 化学结构Chemical Structure
  • 分子式C16H21NO3
  • CAS编号85416-75-7
  • PubChem识别号448055
  • 存放说明Store at +4°C. Store under Desiccating conditions. The product can be stored for up to 12 months.
  • 溶解度概述Soluble in DMSO to 100 mM
  • 处理

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILESCOc2ccc(cc2OC1CCCC1)[C@H]3CC(=O)NC3
  • 来源

    Synthetic

  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab120031 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    (R)-(-)-Rolipram 图像

    • ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    (R)-(-)-Rolipram (ab120031)参考文献

    This product has been referenced in:
    • Souness JE & Scott LC Stereospecificity of rolipram actions on eosinophil cyclic AMP-specific phosphodiesterase. Biochem J 291 ( Pt 2):389-95 (1993). Read more (PubMed: 8387267) »
    • Schneider HH  et al. Stereospecific binding of the antidepressant rolipram to brain protein structures. Eur J Pharmacol 127:105-15 (1986). Read more (PubMed: 3019721) »
    • Wachtel H Neurotropic effects of the optical isomers of the selective adenosine cyclic 3',5'-monophosphate phosphodiesterase inhibitor rolipram in rats in-vivo. J Pharm Pharmacol 35:440-4 (1983). Read more (PubMed: 6136585) »

    See 1 Publication for this product

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