Anti-R Phycoerythrin抗体(Rhodamine) (ab34717)

概述

  • 产品名称
    Anti-R Phycoerythrin抗体(Rhodamine)
    参阅全部 R Phycoerythrin 一抗
  • 描述
    山羊多克隆抗体to R Phycoerythrin (Rhodamine)
  • 偶联物
    Rhodamine. Ex: 550nm, Em: 570nm
  • 特异性
    This antibody recognizes R Phycoerythrin conjugated IgG. It will also cross react with B Phycoerythrin.
  • 经测试应用
    适用于: Immunomicroscopy, Flow Cyt, WBmore details
  • 种属反应性
    Reacts with the seaweed Gracilaria. Not yet tested in other species.
  • 免疫原

    Highly purified R Phycoerythrin from the seaweed Gracilaria.

  • 常规说明

    Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons). Absorption Wavelength: 550 nm. Emission Wavelength: 570 nm. Fluorochrome/Protein Ratio: 4.0 moles TRITC per mole of Goat IgG.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was purified from monospecific antiserun by immunoaffinity chromatography using a R Phycoerythrin coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Immunomicroscopy
Flow Cyt
WB
  • 应用说明
    Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 240 kDa. Reaction with low molecular fragments is typically noted by Western blot.

    This product is also suitable for other antibody based fluorescent assays.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
    • 细胞定位
      Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
    • 别名
      • R phycoerythrin alpha subunit antibody
      • R phycoerythrin beta subunit antibody
      • rpeA antibody
      • rpeB antibody

    文献

    This product has been referenced in:
    • Fujita T  et al. Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep 3:3171 (2013). Read more (PubMed: 24201379) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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