Anti-R Phycoerythrin抗体(Biotin) (ab34730)

概述

  • 产品名称Anti-R Phycoerythrin抗体(Biotin)
    参阅全部 R Phycoerythrin 一抗
  • 描述
    山羊多克隆抗体to R Phycoerythrin (Biotin)
  • 偶联物Biotin
  • 特异性This antibody will cross react with B Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
  • 经测试应用适用于: Dot blot, WB, ELISAmore details
  • 免疫原

    Full length protein Highly purified R Phycoerythrin from the seaweed gracilaria.

  • 常规说明

    10-20 Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) molecules per goat IgG molecule.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
    IgG and Protease free.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/3000. Detects a band of approximately 240 kDa.
ELISA 1/8000 - 1/32000.

靶标

  • 相关性Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
  • 细胞定位Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
  • 别名
    • R phycoerythrin alpha subunit antibody
    • R phycoerythrin beta subunit antibody
    • rpeA antibody
    • rpeB antibody

Anti-R Phycoerythrin antibody (Biotin) (ab34730)参考文献

ab34730 has not yet been referenced specifically in any publications.

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