Anti-R Phycoerythrin抗体(Alkaline Phosphatase) (ab34729)

概述

  • 产品名称Anti-R Phycoerythrin抗体(Alkaline Phosphatase)
    参阅全部 R Phycoerythrin 一抗
  • 描述
    山羊多克隆抗体to R Phycoerythrin (Alkaline Phosphatase)
  • 偶联物Alkaline Phosphatase
  • 经测试应用适用于: Dot blot, ELISA, WBmore details
  • 免疫原

    R-Phycoerythrin from the seaweed gracilaria.

  • 常规说明

    Alkaline Phosphatase (from Calf Intestine, Molecular Weight 140 kDa). Conjugation Reference: Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
ELISA
WB
  • 应用说明ELISA: 1/1,000 - 1/5,000. Assayed against 1.0 ug of R-Phycoerythrin in a standard
    capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
    • 细胞定位Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
    • 别名
      • R phycoerythrin alpha subunit antibody
      • R phycoerythrin beta subunit antibody
      • rpeA antibody
      • rpeB antibody

    Anti-R Phycoerythrin antibody (Alkaline Phosphatase) (ab34729)参考文献

    ab34729 has not yet been referenced specifically in any publications.

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