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RabMAb

Anti-R Cadherin抗体[EPR4836] (ab109242)

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概述

  • 产品名称
    Anti-R Cadherin抗体[EPR4836]
    参阅全部 R Cadherin 一抗
  • 描述
    兔单克隆抗体[EPR4836] to R Cadherin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human R Cadherin aa 800-900. The exact sequence is proprietary.

  • 阳性对照
    • HepG2 cell lysate, fetal brain lysate, SH-SY5Y cell lysate
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 100 kDa.
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC-P.

    • 靶标

    图片

    • Western blot - Anti-R Cadherin antibody [EPR4836] (ab109242)
      Western blot - Anti-R Cadherin antibody [EPR4836] (ab109242)
      All lanes : Anti-R Cadherin antibody [EPR4836] (ab109242) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg/ml per lane.

      Predicted band size: 100 kDa

    文献

    ab109242 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab109242？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    2 reviews or Q&A

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    Votes: Highest First

    We detect bands at 100 and 80 kDa. The blot is generally dark.

    Cell type: mouse osteosarcoma cell lines
    sample loaded: 50ug total protein per lane
    Blocking Buffer:5% non-fat milk with TBST
    Dilution of the primary antibody: 1:6...

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    Thank you for the protocol and sample details.

    The 100kDa band may be cadherin. The size of the protein, without any post-translational modification such as glycosylation, is expected to be 100 kDa, according to the UniProt database entry fo...

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    Hello, I have obtained an antibody (ab109242) from your campany but when I used it for my blotting, what I got was a black backgroud with a ˜100KD band and also a ˜80KD band.Dilution of the antibody was 1:6000.Can you help me to improve my blotting?

    Thank you for bringing this to our attention. Can you please provide some details of your samples and protocol? For instance, what is the species and tissue type or cell type, how were the samples prepared, how much sample did you load per lane, what d...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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