Anti-Pyruvate kinase isozyme M1抗体(ab116271)


  • 产品名称Anti-Pyruvate kinase isozyme M1抗体
    参阅全部 Pyruvate kinase isozyme M1 一抗
  • 描述
    兔多克隆抗体to Pyruvate kinase isozyme M1
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Horse, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to Human Pyruvate kinase isozyme M1 aa 350-450 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab134644)

  • 阳性对照
    • WB: Human fetal heart tissue lysate and HEK293, U87MG, A549, THP1, HepG2 and NIH3T3 whole cell lysates. IHC-P: Human lung adenocarcinoma tissue. ICC/IF: HeLa cells.



Our Abpromise guarantee covers the use of ab116271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.


  • 功能Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • 组织特异性Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 序列相似性Belongs to the pyruvate kinase family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    Under hypoxia, hydroxylated by EGLN3.
  • 细胞定位Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CTHBP antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • OIP-3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • pkm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle isozyme antibody
    • THBP1 antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2-PK antibody
    see all

Anti-Pyruvate kinase isozyme M1 antibody 图像

  • ICC/IF image of ab116271 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab116271, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HepG2 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml.

  • All lanes : Anti-Pyruvate kinase isozyme M1 antibody (ab116271) at 1 µg/ml

    Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 5 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • IHC image of PKM2 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116271, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Pyruvate kinase isozyme M1 antibody (ab116271)参考文献

ab116271 has not yet been referenced specifically in any publications.

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