Pyruvate dehydrogenase (PDH) WB抗体Cocktail (ab110416)

概述

  • 产品名称Pyruvate dehydrogenase (PDH) WB抗体Cocktail
  • 检测类型Quantitative
  • 种属反应性
    与反应: Cow, Human
  • 产品概述

    ab110416 (MSP02) contains a total of 150 µg of monoclonal antibodies reactive against PDH subunits. Isolated mitochondria are available from several species for use as control samples.

    The PDH Western blotting kit contains four different mAbs reacting specifically with E1 alpha, E1 beta, E2 and E2/E3bp. A mAb against the OSCP subunit of ATP synthase is included as a control. This mAb mixture can be used to probe the isolated pyruvate dehydrogenase for integrity and purity.

    Cocktail Antibodies:

    Mouse anti C-V-OSCP [4C11C10D12] monoclonal:
    Amount: 12.5 µg
    Working concentration: 0.5 µg/ml

    Mouse anti PDH-E1-alpha [9H9AF5] monoclonal:
    Amount: 25 µg
    Working concentration: 1 µg/ml

    Mouse anti PDH-E1-beta [17A5E2H8] monoclonal:
    Amount: 62.5 µg
    Working concentration: 2.5 µg/ml

    Mouse anti PDH-E2 [15D3G9C11] monoclonal:
    Amount: 25 µg
    Working concentration: 1 µg/ml

    Mouse anti PDH-E2/E3bp [13G2AE2BH5] monoclonal:
    Amount: 25 µg
    Working concentration: 1 µg/ml

  • 经测试应用适用于: WBmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab110416 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use a concentration of 6 µg/ml. Suggested concentration.

    Pyruvate dehydrogenase (PDH) WB Antibody Cocktail 图像

    • Blocked for overnight with 5% Milk/PBS at 4ºC (or 3 hrs at room temperature).

      Primary antibody incubatedat 6 µg/mL in 1% milk/PBS for 2hrs at room temperature.

      Secondary: HRP-conjugated anti-mouse used according to manufacturers instructions.

      Detection method: ECL+

       

      All wash steps carried out in PBS + 0.05% Tween-20.

    Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416)参考文献

    This product has been referenced in:
    • Ferriero R  et al. Phenylbutyrate therapy for pyruvate dehydrogenase complex deficiency and lactic acidosis. Sci Transl Med 5:175ra31 (2013). Mouse . Read more (PubMed: 23467562) »
    • Donohoe DR  et al. Microbial regulation of glucose metabolism and cell-cycle progression in Mammalian colonocytes. PLoS One 7:e46589 (2012). WB ; Mouse . Read more (PubMed: 23029553) »

    See all 7 Publications for this product

    Product Wall

    Thank you for contacting us.

    Please find the WB protocol used to validate our MitoSciences range of antibodies attached, as well as the pdf datasheets for those products. Keep in mind, we regularly update our online datasheets to reflect the ...

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    Thank you for contacting us regarding the use of ourMitoProfile® Pyruvate dehydrogenase (PDH) WB Antibody Cocktail in rat tissues.

    I have gathered the following information regarding each antibodies reactivty with rat below:

    ...

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    Vielen Dank für die Zusammenfassung in Englisch. Ich habe nun eine Antwort von unseren Mitochondrien -Spezialisten aus dem Labor erhalten.

    Ich habe die Antwort nicht übersetzt um Übersetzungsfehler zu vermeiden:

    "...

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    Thank you for your inquiry.

    I am happy to confirm the molecular weight of the following proteins:

    O (complex 5) : 22kD

    E1beta : 39.4kD

    E1alpha : 43.3kD

    E3bp : 54kD

    E2 : 69kD

    I also a...

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    Thank you for your enquiry. It has taken some time to confirm all the details for you, and I appreciate your patience.

    I am sorry to confirm that MS602 has been discontinued and we do not have anymore in stock.

    Although that produ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"