Pyruvate dehydrogenase (PDH) Profiling ELISA试剂盒(ab110174)

概述

  • 产品名称Pyruvate dehydrogenase (PDH) Profiling ELISA试剂盒
    参阅全部 Pyruvate dehydrogenase 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue
  • 检测类型Sandwich (qualitative)
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 产品概述

    Abcam’s Pyruvate dehydrogenase (PDH) in vitro ProfilingELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the measurement of Pyruvate dehydrogenase (PDH) in Human, bovine, mouse, and rat whole tissue or cell lysate samples.

    Capture antibodies are pre-coated in the wells of modular microplates, which can be broken into 8-well strips. This assay is a “sandwich” ELISA, where the PDH enzyme is purified and immobilized by an anti-PDH capture antibody pre-coated in the microplate wells. The amount of captured PDH is determined by adding a second (detector) anti-PDH antibody which binds to the captured PDH hat a different epitope. This is followed by binding of an HRP conjugated goat anti-mouse antibody that binds the detector anti-PDH antibody. The detector-bound HRP then changes the colorless HRP development solution to blue and the color intensity (absorbance) is proportional to the amount of PDH captured. All of our microplate assays utilize our highly-validated immunocapture antibodies, which are able to capture large, multi-subunit enzyme complexes in their fully intact state.

  • 说明

    5X Stabilizer should be stored at -20°C. Remainder of kit should be stored at 4°C. When stored as recommended the kit is stable for 6 months.

     

    PDH is the key regulatory enzyme of cellular metabolism because it links the TCA cycle and subsequent oxidative phosphorylation with glycolysis and gluconeogenesis as well as with both lipid and amino acid metabolism. PDH activity is regulated primarily by PDK-dependent phosphorylation and PDP-dependent dephosphorylation of PDH. Phosphorylation inactivates PDH whereas dephosphorylation activates PDH. Phosphorylation occurs at Serines 232, 293, and 300 of the human E1α subunits.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 别名
    • PDC
    • PDH
    • PDHC

应用

Our Abpromise guarantee covers the use of ab110174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Pyruvate dehydrogenase (PDH) Profiling ELISA Kit 图像

  • An example of the quantity of PDH capture from a HepG2 cultured cell lysate. The sample was diluted to show that over this range of concentrations that can be used. Each sample was measured in 6 replicates. Bars show standard deviations.
  • Abcams' protein quantity microplate assays use the well-established sandwich ELISA format, whereby capture and detector antibodies are used to immobilize and then quantify a target protein or enzyme. All of our microplate assays utilize our highly-validated immunocapture antibodies, which are able to capture large, multi-subunit enzyme complexes in their fully intact state. Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, a second monoclonal antibody, against a different epitope on the target, is added to the well. This detector antibody is either directly labeled with biotin, or a biotin-labeled goat anti-mouse secondary is added. Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.

实验方案

Pyruvate dehydrogenase (PDH) Profiling ELISA Kit (ab110174)参考文献

This product has been referenced in:
  • Zager RA  et al. Renal Cortical Pyruvate Depletion during AKI. J Am Soc Nephrol N/A:N/A (2014). ELISA ; Mouse . Read more (PubMed: 24385590) »
  • Yoshioka J  et al. Deletion of thioredoxin-interacting protein in mice impairs mitochondrial function but protects the myocardium from ischemia-reperfusion injury. J Clin Invest 122:267-79 (2012). Read more (PubMed: 22201682) »

See all 2 Publications for this product

Product Wall

To answer your previous questions:

1) To which subunit does the antibody bind?
E2/E3bp

2) Does the antibody recognize a linear epitope or a conformational epitope?
Conformational epitope

3) Does the antibody bind to t...

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Thank you very much for your call last week and for your patience while I have been in touch with the lab about this kit.

The antibodies used in the kit were actually raised against the pig protein,so we expect it to work with the pig protein...

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Thank you for contacting ABcam in regards to the lysis buffer that your tissues are in and how that will work with our PDH kit.

These kits will not function well with samples in such a harsh buffer. It is recommended that samples are in PSB ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"