Pyruvate dehydrogenase (PDH) Enzyme Activity试纸检测试剂盒(ab109882)


  • 产品名称
    Pyruvate dehydrogenase (PDH) Enzyme Activity试纸检测试剂盒
    参阅全部 Pyruvate dehydrogenase 试剂盒
  • 样品类型
    Cell culture extracts, Tissue
  • 检测类型
    Sandwich (quantitative)
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Pig
  • 产品概述

    ab109882 is used to quantify the activity of the PDH enzyme complex from human, bovine, mouse, rat and pig samples. This is accomplished by immunocapturing PDH with an anti-PDH antibody immobilized on a defined section of a dipstick. The enzyme complex is immunocaptured in its native form and activity is measured by the reaction scheme. PDH activity is visualized by coupling PDH-dependent production of NADH to the reduction of NBT in the presence of excess diaphorase, forming an insoluble intensely colored precipitate at the capture line. The signal intensity is measured by a dipstick reader or analyzed by other imaging systems such as a flatbed scanner.

    Purified mitochondria can be used in this assay. However, homogenized tissue and whole cells can also be used without the need for mitochondrial isolation.

    Pyruvate dehydrogenase activity is regulated by PDH kinase and PDH phosphatase. Cells grown in glucose media derive most of their energy from glycolysis; therefore, most of their PDH complex may be in an inactive phosphorylated form at the time of isolation. Growing cells in alternate carbon sources such as glutamine/galactose may up-regulate PDH activity. This kit does not include PDH kinases, PDH phosphatases or their respective inhibitors. These may be incorporated into the assay at the user’s discretion.

  • 说明

    Store dipsticks at room temperature out of direct sunlight in their provided desiccant container. Avoid high humidity conditions.

    Store Sample buffer at 4°C.

    Store 20X Reagent Mix, Detergent, and Diaphorase at -80°C, but these may be aliquoted upon receipt to prevent freeze/thaw cycles.

    Store Blocking solution at -20°C.

    Store NBT in the dark at -80°C.

  • 经测试应用
    适用于: Functional Studiesmore details
  • 平台



Our Abpromise guarantee covers the use of ab109882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.


  • Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.
  • Figure 1. An example using the ab109882 to measure PDH activity in human fibroblast sample. Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 160 µg of fibroblast protein extract in a 100 µL reaction volume (50 µL sample in Sample buffer plus 50 µL of Blocking solution). The dipsticks were developed for 1 hour in Activity buffer.
  • Figure 2. An example using the ab109882 to measure PDH activity in human fibroblast sample. Based on the standard curve, 160 µg of protein extract were loaded onto a dipstick for each sample. The figure shows two developed dipsticks, a control sample and a sample from a patient with a mutation in the E1-alpha subunit of PDH. The analysis of the signal intensity and interpolation from the standard curve showed that the patient sample had 8% the PDH activity of the control.



This product has been referenced in:
  • Goguet-Rubio P  et al. E4F1-mediated control of pyruvate dehydrogenase activity is essential for skin homeostasis. Proc Natl Acad Sci U S A 113:11004-9 (2016). Read more (PubMed: 27621431) »
  • Lacroix M  et al. E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity. Proc Natl Acad Sci U S A 113:10998-1003 (2016). Read more (PubMed: 27621446) »

See all 12 Publications for this product


I reviewed details of the instrument on this page with the laboratories:

It may work to read this assay, but to be sure the best would be to ask BioRad technical support to see if their instrument c...

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There are a few different means of interpreting the results with our dipstick assays.

1) You can do relative quantification in which you do not need a dipstick reader. You would simply be visualizing the relative intensity and comparing betwee...

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I can confirm that either scrapping or enzymatic digestion with trypsin are acceptable methods for harvesting the cultured cells.

We haven't tested the kit ab109882 with yeast samples so I am sorry we are unable to confirm the species cross reactivity.

None of the anti PDH antibody we have is tested with yeast sample so it is difficult for us to comment on the cross reac...

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DCA or any other inhibitors of PDH kinases are not in any of the kit’s components. Also, no phosphatase inhibitors are present.

Thank you for your response.

You can use regular scanner to scan and analyze your result with image J software. I would suggest you to follow your plan, i.e. purchase a kit and check whether it is sensitive enough for your sample types.

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Thank you for your response.

There is an instrument called "Dipstick Reader" which has been developed to read the signal. The Protocol Booklet and Measurement Program Instruction Manual can be downloaded through the website:
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Thank you for contacting us.

Please find attached the sample preparation guide we have.

10 million cells/ ml give 5 mg of protein/ ml. You can easily scale down the volume and number of cells.

You would only need 25 ul of sam...

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Thank you for your enquiry.

We have not tested Arabidopsis with this kit. The immunogen used to generate the capture antibody in this kit was Purified PDH protein from Pig Heart. Therefore, we are unable to predict homology with your species...

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Thank you for contacting us.

With regards to ab109720, there is a publication on the utility of Complex I enzyme activity dipsticks in patients with specific mutations in the different subunits of complex I. The antibody stripped to the nitr...

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