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Porcine Pyruvate dehydrogenase E2/E3bp.
Our Abpromise guarantee covers the use of ab110333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 54 , 69 kDa.|
|ICC/IF||Use a concentration of 1 µg/ml. (heat-induced antigen-retrieval improves signal)|
|Flow Cyt||Use a concentration of 1 mg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-P||1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
ab110333 staining Pyruvate dehydrogenase E2/E3bp in Human HUVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour. A CF568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.