Anti-Pyruvate Dehydrogenase E1 beta subunit抗体(ab84588)

概述

  • 产品名称Anti-Pyruvate Dehydrogenase E1 beta subunit抗体
    参阅全部 Pyruvate Dehydrogenase E1 beta subunit 一抗
  • 描述
    兔多克隆抗体to Pyruvate Dehydrogenase E1 beta subunit
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (GLWKKYGDKR IIDTPISEMG FAGIAVGAAM AGLRPICEFM TFNFSMQAID) of Human Pyruvate Dehydrogenase E1 beta subunit (NP_000916).

  • 阳性对照
    • Human fetal heart lysate.

应用

Our Abpromise guarantee covers the use of ab84588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 疾病相关Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody 图像

  • Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab84588) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab84588)参考文献

ab84588 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.
As long as your mutant construct contains the either amino acid regions 72-121 or 250-360, then you should be able to either ab84588 orab55574, as these antibodies will bind within these regions respectively.
Whether...

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Thank you for contacting Abcam.
Depending on the exact amino acids that are missing from your mutant construct, one of the Pyruvate Dehydrogenase E1 beta subunit antibodies we have should work for you. Below is the immunogen information for each of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"