Anti-Pyruvate Dehydrogenase E1 beta subunit抗体[17A5E2H8 ] (ab110331)


  • 产品名称Anti-Pyruvate Dehydrogenase E1 beta subunit抗体[17A5E2H8 ]
    参阅全部 Pyruvate Dehydrogenase E1 beta subunit 一抗
  • 描述
    小鼠单克隆抗体[17A5E2H8 ] to Pyruvate Dehydrogenase E1 beta subunit
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Porcine Pyruvate Dehydrogenase E1 beta subunit protein.

  • 阳性对照
    • Isolated mitochondria from Human, Bovine, Rat and Mouse hearts; HepG2 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal duodenum.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.




Our Abpromise guarantee covers the use of ab110331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.


  • 功能The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 疾病相关Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] 图像

  • IHC image of Pyruvate Dehydrogenase E1 beta subunit staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110331, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 10 µg/ml
    Lane 2 : Isolated mitochondria from Bovine heart at 4 µg/ml
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg/ml
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg/ml
    Lane 5 : HepG2 cell lysate at 20 µg/ml

    Predicted band size : 39 kDa

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331)参考文献

This product has been referenced in:
  • Halim ND  et al. Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons. Glia 58:1168-76 (2010). Read more (PubMed: 20544852) »
  • Han Z  et al. A combined therapeutic approach for pyruvate dehydrogenase deficiency using self-complementary adeno-associated virus serotype-specific vectors and dichloroacetate. Mol Genet Metab 93:381-7 (2008). Read more (PubMed: 18206410) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (keratinocytes mitochondria fraction)
Loading amount 66 µg
Specification keratinocytes mitochondria fraction
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Mar 26 2013

Thank you for your reply.
As long as your mutant construct contains the either amino acid regions 72-121 or 250-360, then you should be able to either ab84588 orab55574, as these antibodies will bind within these regions respectively.

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Thank you for contacting Abcam.
Depending on the exact amino acids that are missing from your mutant construct, one of the Pyruvate Dehydrogenase E1 beta subunit antibodies we have should work for you. Below is the immunogen information for each of...

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Thank you for calling Abcam.

I am sorry that you were having issues with ab110331 in western blot. As requested I have sent you ab55574 as a free of charge replacement, your new order number is ********.

Please let me know if there ...

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