Anti-Pyruvate Dehydrogenase E1-alpha subunit抗体(ab110474)


  • 产品名称
    Anti-Pyruvate Dehydrogenase E1-alpha subunit抗体
    参阅全部 Pyruvate Dehydrogenase E1-alpha subunit 一抗
  • 描述
    兔多克隆抗体to Pyruvate Dehydrogenase E1-alpha subunit
  • 特异性
    ab110474 detects endogenous levels of total Pyruvate Dehydrogenase E1-alpha subunit protein.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal region of Human Pyruvate Dehydrogenase E1-alpha subunit (UniProt ID: P08559).

  • 阳性对照
    • WB: Extracts from A549, HepG2 and Jurkat cells IHC-P: Human breast carcinoma tissue



Our Abpromise guarantee covers the use of ab110474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is heat-mediated, citrate buffer pH 6.0. A pressure cooker was used for about 5 minutes.


  • 功能
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 组织特异性
  • 疾病相关
    Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ODPA_HUMAN antibody
    • PDH antibody
    • PDHA antibody
    • PDHA1 antibody
    • PDHCE1A antibody
    • PDHE1 A type I antibody
    • PDHE1-A type I antibody
    • PHE1A antibody
    • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
    • Pyruvate Dehydrogenase E1 alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
    see all

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody 图像

  • All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab110474) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from A549 cells
    Lane 4 : Extracts from Jurkat cells, treated with the synthesized peptide

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 43 kDa
  • ab110474, at a 1/50 dilution, staining Pyruvate Dehydrogenase E1-alpha subunit in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the synthesized peptide.

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab110474)参考文献

ab110474 has not yet been referenced specifically in any publications.

Product Wall

Yes, ab110474 was run under reduced, denatured conditions.

Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Mouse Tissue sections (mouse brain tissue sections)
mouse brain tissue sections
Yes - 0.3% of triton-X 100

Qian Ma

Verified customer

提交于 Jan 03 2014

I am very pleased to hear you would like to accept our offer and test ab110474 in mouse. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the...

Read More