Anti-Pyruvate Dehydrogenase E1-alpha subunit抗体[9H9AF5] (ab110330)

概述

性能

应用

Our Abpromise guarantee covers the use of ab110330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 5 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

In-Cell ELISA Use a concentration of 8 µg/ml.

靶标

  • 功能
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 组织特异性
    Ubiquitous.
  • 疾病相关
    Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ODPA_HUMAN antibody
    • PDH antibody
    • PDHA antibody
    • PDHA1 antibody
    • PDHCE1A antibody
    • PDHE1 A type I antibody
    • PDHE1-A type I antibody
    • PHE1A antibody
    • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
    • Pyruvate Dehydrogenase E1 alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
    see all

图片

  • Immunocytochemistry analysis using ab110330 at 5µg/ml staining Pyruvate Dehydrogenase E1-alpha subunit in cultured, normal Human embryonic lung fibroblasts (strain MRC5) followed by AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 µg/ml).
  • Immunocytochemical analysis of SH-SY5Y human neuroblastoma labeling Pyruvate dehydrogenase E1-alpha with ab110330 at 1/500 dilution.

    See Abreview

  • Flow cytometric analysis using ab110330 at 1µg/ml staining Pyruvate Dehydrogenase E1-alpha subunit in HL60 cells (blue). Isotype control antibody (red).
  • All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5] (ab110330) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 10 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : hepG2 cell lysate at 20 µg


    Predicted band size : 43 kDa

文献

This product has been referenced in:
  • Zhang Y  et al. Regulation of hepatic pyruvate dehydrogenase phosphorylation in offspring glucose intolerance induced by intrauterine hyperglycemia. Oncotarget 8:15205-15212 (2017). Read more (PubMed: 28148899) »
  • Schatton D  et al. CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J Cell Biol 216:675-693 (2017). WB ; Mouse . Read more (PubMed: 28188211) »

See all 28 Publications for this product

客户评价及客户问答

Thank you for your enquiry,

I am sorry to confirm that this kit is no longer available, and apologise for the inconvenience.

I can confirm that we do sell the separate antibody components which you may like to consider:

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Application
Western blot
Sample
Sheep Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Jennifer Bruce

Verified customer

提交于 Jun 12 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (Ovarian cancer cell lines)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Ovarian cancer cell lines
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 25 2015

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Mouse Tissue lysate - whole (heart)
Specification
heart
Total protein in input
1000 µg
Username

Abcam user community

Verified customer

提交于 Feb 23 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Human Cell (SH-SY5Y human neuroblastoma)
Specification
SH-SY5Y human neuroblastoma
Permeabilization
Yes - 0.2% NP-40, 3min
Fixative
Formaldehyde
Username

Dr. George Allen

Verified customer

提交于 Dec 16 2013

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Please find the WB protocol used to validate our MitoSciences range of antibodies attached, as well as the pdf datasheets for those products. Keep in mind, we regularly update our online datasheets to reflect the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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