兔多克隆抗体to Pyrophosphatase 1
Mouse, Rat, Cow, Monkey
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 259 of Human Pyrophosphatase 1 (UniProt ID: Q15181).
293T, Raji. A431, H1299, HeLa, HepG2, MOLT4 whole cell lysates for WB. HeLa cells for IF. OVCAR3 xenograft for IHC.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
1/100 - 1/500.
1/100 - 1/200.
Belongs to the PPase family.
Information by UniProt
cytosolic inorganic pyrophosphatase antibody
diphosphate phosphohydrolase antibody
inorganic diphosphatase antibody
Western blot - Anti-Pyrophosphatase 1 antibody (ab96099)
All lanes : Anti-Pyrophosphatase 1 antibody (ab96099) at 1/10000 dilution Lane 1 : H1299 Whole cell lysate Lane 2 : HeLa Whole cell lysate Lane 3 : HepG2 Whole cell lysate Lane 4 : MOLT4 Whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 32 kDa 12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Pyrophosphatase 1 antibody (ab96099)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96099 at 1/200 dilution. Lower image merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyrophosphatase 1 antibody (ab96099)
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ab96099 at 1/500 dilution.
This product has been referenced in:
et al. Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism. JPEN J Parenter Enteral Nutr N/A:N/A (2016).
Read more (PubMed: 27503937) »
et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013).
Read more (PubMed: 23462207) »
See all 4 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"