概述

  • 产品名称Anti-Pyrophosphatase 1抗体
    参阅全部 Pyrophosphatase 1 一抗
  • 描述
    兔多克隆抗体to Pyrophosphatase 1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human, Pig
    预测可用于: Mouse, Rat, Cow, Monkey
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 259 of Human Pyrophosphatase 1 (UniProt ID: Q15181).

  • 阳性对照
    • 293T, Raji. A431, H1299, HeLa, HepG2, MOLT4 whole cell lysates for WB. HeLa cells for IF. OVCAR3 xenograft for IHC.

性能

应用

Our Abpromise guarantee covers the use of ab96099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

Anti-Pyrophosphatase 1 antibody 图像

  • All lanes : Anti-Pyrophosphatase 1 antibody (ab96099) at 1/10000 dilution

    Lane 1 : H1299 Whole cell lysate
    Lane 2 : HeLa Whole cell lysate
    Lane 3 : HepG2 Whole cell lysate
    Lane 4 : MOLT4 Whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 32 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96099 at 1/200 dilution. Lower image merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ab96099 at 1/500 dilution.

Anti-Pyrophosphatase 1 antibody (ab96099)参考文献

This product has been referenced in:
  • Megger DA  et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013). Read more (PubMed: 23462207) »
  • Yi YJ  et al. Identification of the inorganic pyrophosphate metabolizing, ATP substituting pathway in mammalian spermatozoa. PLoS One 7:e34524 (2012). WB, ICC/IF ; Pig . Read more (PubMed: 22485177) »

See all 3 Publications for this product

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