Anti-Pyrophosphatase 1抗体(ab96099)
- 数据表
- 具体的参考文献 (4)
- 实验方案
概述
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产品名称Anti-Pyrophosphatase 1抗体
参阅全部 Pyrophosphatase 1 一抗 -
描述兔多克隆抗体to Pyrophosphatase 1
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宿主Rabbit
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经测试应用适用于: WB, IHC-P, ICC/IFmore details
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种属反应性与反应: Human, Pig
预测可用于: Mouse, Rat, Cow, Monkey
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免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 259 of Human Pyrophosphatase 1 (UniProt ID: Q15181).
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阳性对照
- 293T, Raji. A431, H1299, HeLa, HepG2, MOLT4 whole cell lysates for WB. HeLa cells for IF. OVCAR3 xenograft for IHC.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab96099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 32 kDa. | |
| IHC-P | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/200. |
靶标
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组织特异性Expressed ubiquitously.
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序列相似性Belongs to the PPase family.
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细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 280701 Cow
- Entrez Gene: 5464 Human
- Entrez Gene: 67895 Mouse
- Entrez Gene: 294504 Rat
- Omim: 179030 Human
- SwissProt: P37980 Cow
- SwissProt: Q15181 Human
- SwissProt: Q9D819 Mouse
see all -
别名
- cytosolic inorganic pyrophosphatase antibody
- diphosphate phosphohydrolase antibody
- inorganic diphosphatase antibody
see all
图片
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Western blot - Anti-Pyrophosphatase 1 antibody (ab96099)All lanes : Anti-Pyrophosphatase 1 antibody (ab96099) at 1/10000 dilution
Lane 1 : H1299 Whole cell lysate
Lane 2 : HeLa Whole cell lysate
Lane 3 : HepG2 Whole cell lysate
Lane 4 : MOLT4 Whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Pyrophosphatase 1 antibody (ab96099)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96099 at 1/200 dilution. Lower image merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyrophosphatase 1 antibody (ab96099)Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ab96099 at 1/500 dilution.
实验方案
文献
This product has been referenced in:
- Jiang P et al. Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism. JPEN J Parenter Enteral Nutr N/A:N/A (2016). Read more (PubMed: 27503937) »
- Megger DA et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013). Read more (PubMed: 23462207) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"