Anti-PYK2 (phospho Y881)抗体(ab4801)


  • 产品名称Anti-PYK2 (phospho Y881)抗体
    参阅全部 PYK2 一抗
  • 描述
    兔多克隆抗体to PYK2 (phospho Y881)
  • 特异性Some cross-reactivity with Focal Adhesion Kinase phosphorylated at tyrosine 925 (the corresponding Grb 2 binding site) may be observed in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 in the same cell system.
  • 经测试应用适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Chicken, Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 881.

  • 阳性对照
    • Chick embryo fibroblast (CEF) cells expressing human PYK 2.



Our Abpromise guarantee covers the use of ab4801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明
    IHC-Fr: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with PYK 2 (phospho) antibody panel or CEF whole cell lysate, positive / negative for PYK 2 (pair).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
    • 组织特异性Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
    • 细胞定位Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CADTK antibody
      • CAK-beta antibody
      • CAKB antibody
      • CAKbeta antibody
      • Calcium regulated non receptor proline rich tyrosine kinase antibody
      • Calcium-dependent tyrosine kinase antibody
      • Cell adhesion kinase beta antibody
      • E430023O05Rik antibody
      • EC antibody
      • FADK 2 antibody
      • FADK2 antibody
      • FAK2 antibody
      • FAK2_HUMAN antibody
      • Focal adhesion kinase 2 antibody
      • MGC124628 antibody
      • PKB antibody
      • Proline-rich tyrosine kinase 2 antibody
      • Protein kinase B antibody
      • Protein Tyrosine Kinase 2 Beta antibody
      • Protein-tyrosine kinase 2-beta antibody
      • PTK antibody
      • PTK2B antibody
      • PTK2B protein tyrosine kinase 2 beta antibody
      • PYK2 antibody
      • RAFTK antibody
      • RAFTK2 antibody
      • Related adhesion focal tyrosine kinase antibody
      see all

    Anti-PYK2 (phospho Y881) antibody 图像

    • Predicted band size : 116 kDa

      Peptide Competition: Extracts prepared from CEF cells expressing Pyk2 and plated on fibronectin were resolved by SDS-PAGE on a 10% Tris glycine gel. The proteins were then transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.75 µg/mL ab4801 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the peptide immunogen (2), the non-phosphopeptide corresponding to the Pyk2 phosphopeptide (3), the phosphopeptide corresponding to the tyrosine 861 of the FAK protein (4), and a generic phosphotyrosine peptide (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the phosphopeptide corresponding to ab4801 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

    Anti-PYK2 (phospho Y881) antibody (ab4801)参考文献

    ab4801 has not yet been referenced specifically in any publications.

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