Anti-PYK2 (phospho Y580)抗体(ab52209)


  • 产品名称Anti-PYK2 (phospho Y580)抗体
    参阅全部 PYK2 一抗
  • 描述
    兔多克隆抗体to PYK2 (phospho Y580)
  • 特异性ab52209 detects endogenous levels of PYK2 only when phosphorylated at tyrosine 580.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human PYK2 around the phosphorylation site of tyrosine 580 (D-Y-YP-K-A).

  • 阳性对照
    • Human brain tissue, extracts from K562 cells.



Our Abpromise guarantee covers the use of ab52209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.


  • 功能Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • 组织特异性Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • 细胞定位Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CADTK antibody
    • CAK-beta antibody
    • CAKB antibody
    • CAKbeta antibody
    • Calcium regulated non receptor proline rich tyrosine kinase antibody
    • Calcium-dependent tyrosine kinase antibody
    • Cell adhesion kinase beta antibody
    • E430023O05Rik antibody
    • EC antibody
    • FADK 2 antibody
    • FADK2 antibody
    • FAK2 antibody
    • FAK2_HUMAN antibody
    • Focal adhesion kinase 2 antibody
    • MGC124628 antibody
    • PKB antibody
    • Proline-rich tyrosine kinase 2 antibody
    • Protein kinase B antibody
    • Protein Tyrosine Kinase 2 Beta antibody
    • Protein-tyrosine kinase 2-beta antibody
    • PTK antibody
    • PTK2B antibody
    • PTK2B protein tyrosine kinase 2 beta antibody
    • PYK2 antibody
    • RAFTK antibody
    • RAFTK2 antibody
    • Related adhesion focal tyrosine kinase antibody
    see all

Anti-PYK2 (phospho Y580) antibody 图像

  • This image shows human brain tissue stained with ab52209 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.
  • All lanes : Anti-PYK2 (phospho Y580) antibody (ab52209) at 1/500 dilution

    Lane 1 : Extracts from K562 cells with no immunising peptide
    Lane 2 : Extracts from K562 cells with immunising peptide

    Predicted band size : 116 kDa

Anti-PYK2 (phospho Y580) antibody (ab52209)参考文献

ab52209 has not yet been referenced specifically in any publications.

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Ich habe die Testdatendes Antikörpers in ELISA mit dem Phospho-Peptid und dem un-phosphorylierten Peptid als Anhang zugefügt. ab52209 zeigt eine deutl...

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