Anti-PYK2 (phospho Y579 + Y580)抗体(ab4807)

概述

  • 产品名称Anti-PYK2 (phospho Y579 + Y580)抗体
    参阅全部 PYK2 一抗
  • 描述
    兔多克隆抗体to PYK2 (phospho Y579 + Y580)
  • 特异性Some cross-reactivity still may be experienced in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 within the same cell system.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Chicken, Human
  • 免疫原

    Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosines 579 and 580. The sequence is conserved in human and rat.

  • 阳性对照
    • Vandate treated CEF cells expressing PYK 2 and plated on fibronectin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2, (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence, and (iii) a phosphopeptide derived from the corresponding region of Focal Adhesion Kinase (a PYK 2-related protein) to remove antibody that is reactive with phosphorylated Focal Adhesion Kinase protein. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosines 579 and 580.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with PYK2 peptide - phospho Y579 + Y580 (phospho and non-phospho pair) or CEF whole cell lysate, positive / negative for PYK 2 (pair).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
    • 组织特异性Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
    • 细胞定位Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CADTK antibody
      • CAK-beta antibody
      • CAKB antibody
      • CAKbeta antibody
      • Calcium regulated non receptor proline rich tyrosine kinase antibody
      • Calcium-dependent tyrosine kinase antibody
      • Cell adhesion kinase beta antibody
      • E430023O05Rik antibody
      • EC 2.7.10.2 antibody
      • FADK 2 antibody
      • FADK2 antibody
      • FAK2 antibody
      • FAK2_HUMAN antibody
      • Focal adhesion kinase 2 antibody
      • MGC124628 antibody
      • PKB antibody
      • Proline-rich tyrosine kinase 2 antibody
      • Protein kinase B antibody
      • Protein Tyrosine Kinase 2 Beta antibody
      • Protein-tyrosine kinase 2-beta antibody
      • PTK antibody
      • PTK2B antibody
      • PTK2B protein tyrosine kinase 2 beta antibody
      • PYK2 antibody
      • RAFTK antibody
      • RAFTK2 antibody
      • Related adhesion focal tyrosine kinase antibody
      see all

    Anti-PYK2 (phospho Y579 + Y580) antibody 图像



    • Predicted band size : 116 kDa


      Peptide Competition: Cell extracts prepared from chick embryo fibroblasts treated with vanadate were plated on fibronectin with (lanes 1-5) or without (lane 6) transfection of a PYK 2 expression vector and resolved by SDS PAGE on a 10% Tris-glycine gel. The proteins were then transferred to nitrocellulose. Membranes were incubated with 0.50 µg/mL ab4807, following prior incubation in the presence of the phosphopeptide immunogen (1), the absence of the phosphopeptide immunogen (2, 6), the non phosphopeptide corresponding to the PYK 2 phosphopeptide (3), the phosphopeptide corresponding to PYK 2 [pY579] (4), and the phosphopeptide corresponding to PYK 2 [pY580] (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the dual-phosphopeptide corresponding to this site blocks the antibody signal, not the corresponding mono phosphopeptides, thereby demonstrating the specificity of ab4807 for the dually phosphorylated epitope.

    Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)参考文献

    ab4807 has not yet been referenced specifically in any publications.

    Product Wall

    On gel systems that resolve FAK (pp125) and Pyk2 (pp116), they will show up as a distinct doublet. If there is a lot of FAK relative to Pyk2, the normally low level of cross-reactivity (due to the fact that the antibodies are negatively adsorbed with t...

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