Anti-PYK2 (phospho Y579)抗体(ab78209)


  • 产品名称Anti-PYK2 (phospho Y579)抗体
    参阅全部 PYK2 一抗
  • 描述
    兔多克隆抗体to PYK2 (phospho Y579)
  • 特异性This antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 579.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthesized phosphopeptide derived from human PYK2 around the phosphorylation site of tyrosine 579 (E-D-YP-Y-K). (Peptide available as ab135942.)

  • 阳性对照
    • Extracts from 3T3 cells. Human brain tissue.




Our Abpromise guarantee covers the use of ab78209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).Can be blocked with Human PYK2 (phospho T579) peptide (ab135942).
IHC-P 1/50 - 1/100.
ELISA 1/20000.


  • 功能Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • 组织特异性Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • 细胞定位Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CADTK antibody
    • CAK-beta antibody
    • CAKB antibody
    • CAKbeta antibody
    • Calcium regulated non receptor proline rich tyrosine kinase antibody
    • Calcium-dependent tyrosine kinase antibody
    • Cell adhesion kinase beta antibody
    • E430023O05Rik antibody
    • EC antibody
    • FADK 2 antibody
    • FADK2 antibody
    • FAK2 antibody
    • FAK2_HUMAN antibody
    • Focal adhesion kinase 2 antibody
    • MGC124628 antibody
    • PKB antibody
    • Proline-rich tyrosine kinase 2 antibody
    • Protein kinase B antibody
    • Protein Tyrosine Kinase 2 Beta antibody
    • Protein-tyrosine kinase 2-beta antibody
    • PTK antibody
    • PTK2B antibody
    • PTK2B protein tyrosine kinase 2 beta antibody
    • PYK2 antibody
    • RAFTK antibody
    • RAFTK2 antibody
    • Related adhesion focal tyrosine kinase antibody
    see all

Anti-PYK2 (phospho Y579) antibody 图像

  • All lanes : Anti-PYK2 (phospho Y579) antibody (ab78209) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells
    Lane 2 : Extracts from 3T3 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 116 kDa
    Observed band size : 116 kDa
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78209 at a 1/50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

Anti-PYK2 (phospho Y579) antibody (ab78209)参考文献

ab78209 has not yet been referenced specifically in any publications.

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ab135942 (or us...

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