兔多克隆抗体to Pygopus 2
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 356-406 (RECTGMTESA YGLLTTEASA VWACDLCLKT KEIQSVYIRE GMGQLVAAND G) of Human Pygopus 2 (NP_612157.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Involved in signal transduction through the Wnt pathway.
Contains 1 PHD-type zinc finger.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pygopus 2 antibody (ab99275)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Pygopus 2 with ab99275 at 1/1000 (1µg/mg). Detection: DAB.
Immunoprecipitation - Pygopus 2 antibody (ab99275)
Detection of Pygopus 2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99275 at 3 µg/mg lysate for IP (Lane 1). An anti-Pygopus 2 antibody, which recognizes an upstream epitope, was used at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
has not yet been referenced specifically in any publications.
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