概述

  • 产品名称Anti-PYGM抗体
    参阅全部 PYGM 一抗
  • 描述
    小鼠单克隆抗体to PYGM
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 734-843 of Human PYGM with a 26kDa tag. NP_005600.

  • 阳性对照
    • Recombinant protein, PC12 cell lysate, HepG2 cell lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab88078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 97 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • 疾病相关Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) [MIM:232600]; also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
  • 序列相似性Belongs to the glycogen phosphorylase family.
  • 翻译后修饰Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glycogen phosphorylase antibody
    • Glycogen phosphorylase muscle form antibody
    • muscle form antibody
    • Muscpho antibody
    • Myophosphorylase antibody
    • Phosphorylase glycogen muscle (McArdle syndrome glycogen storage disease type V) antibody
    • Pygm antibody
    • PYGM_HUMAN antibody
    see all

Anti-PYGM antibody 图像

  • All lanes : Anti-PYGM antibody (ab88078) at 1/400 dilution

    Lane 1 : Mouse liver whole tissue lysate
    Lane 2 : Mouse thigh muscle whole tissue lysate
    Lane 3 : Mouse dendritic whole cell lysate
    Lane 4 : HEK293 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated rabbit anti-mouse IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 97 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 2% milk for 1 hour at room temperature.

    Incubated with the primary antibody for 12 hours at 4°C in blocking buffer.

    See Abreview

  • Anti-PYGM antibody (ab88078) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 97 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant fragment) was used for the test lane.
  • Anti-PYGM antibody (ab88078) at 1 µg/ml + PC12 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Anti-PYGM antibody (ab88078) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Anti-PYGM antibody (ab88078) at 1 µg/ml + Raw264.7 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Anti-PYGM antibody (ab88078) at 1 µg/ml + NIH/3T3 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa

Anti-PYGM antibody (ab88078)参考文献

This product has been referenced in:
  • Garvey SM  et al. Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats. Physiol Rep 3:N/A (2015). WB ; Rat . Read more (PubMed: 25716928) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Thigh muscle)
Gel Running Conditions Reduced Denaturing (SDS-PAGE 12% gel)
Loading amount 30 µg
Specification Thigh muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 22 2015

The PYGL antibody ab103419 is raised against the full-length protein, amino acids 1-847. The sequence is described in the UniProt entry, accession P06737. Here is a link to the entry. http://www.uniprot.org/uniprot/P06737.

It is a polyclonal a...

Read More
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Tissue lysate - whole (skeletal muscle)
Specification skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rafael Montes Nieto

Verified customer

提交于 Oct 30 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 03 2014

Thank you for your enquiry and your interest in our products.
The homology between mouse and human seems to be very high (above 95%) so cross-reactivity with murine PYGM can be expected even for those two antibodies (ab63158 and ab63159) which hav...

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Thank you for your call yesterday and for your patience while I have looked throughsome literatureregarding glycogen phosphorylase.

As we discussed, it might be possible to distinguish the active and inactive (phosphorylated and unphosphoryla...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"