概述

  • 产品名称Anti-PYGM抗体
    参阅全部 PYGM 一抗
  • 描述
    兔多克隆抗体to PYGM
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH from the C terminal region of human PYGM.

  • 阳性对照
    • WB: 293 cell lysate. IHC-P: Human skeletal muscle.

性能

应用

Our Abpromise guarantee covers the use of ab63158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

靶标

  • 功能Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • 疾病相关Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) [MIM:232600]; also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
  • 序列相似性Belongs to the glycogen phosphorylase family.
  • 翻译后修饰Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glycogen phosphorylase antibody
    • Glycogen phosphorylase muscle form antibody
    • muscle form antibody
    • Muscpho antibody
    • Myophosphorylase antibody
    • Phosphorylase glycogen muscle (McArdle syndrome glycogen storage disease type V) antibody
    • Pygm antibody
    • PYGM_HUMAN antibody
    see all

Anti-PYGM antibody 图像

  • ab63158, at a 1/10 dilution, staining PYGM in formalin fixed, paraffin embedded human skeletal muscle tissue by Immunohistochemistry. ab63158 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • All lanes : Anti-PYGM antibody (ab63158) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : Lysates of 293 cells transiently transfected with PYGM gene.

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 97 kDa
    Observed band size : 97 kDa

Anti-PYGM antibody (ab63158)参考文献

This product has been referenced in:
  • Nogales-Gadea G  et al. Expression of Glycogen Phosphorylase Isoforms in Cultured Muscle from Patients with McArdle's Disease Carrying the p.R771PfsX33 PYGM Mutation. PLoS One 5: (2010). WB ; Human . Read more (PubMed: 20957198) »

See 1 Publication for this product

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The homology between mouse and human seems to be very high (above 95%) so cross-reactivity with murine PYGM can be expected even for those two antibodies (ab63158 and ab63159) which hav...

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