概述
-
产品名称Anti-PXR抗体
参阅全部 PXR 一抗 -
描述山羊多克隆抗体to PXR
-
经测试应用适用于: WB, IHC-Pmore details
-
种属反应性与反应: Human
预测可用于: Cynomolgus monkey
-
免疫原
Synthetic peptide:
EQFAITLKSYIECNR
, corresponding to internal sequence amino acids 367-381 of Human PXR (NP_003880.3; NP_071285.1; NP_148934.1). -
阳性对照
- HepG2 cell lysate, Human small intestine tissue
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
-
Positive Controls
应用
Our Abpromise guarantee covers the use of ab109728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa). | |
| IHC-P | Use a concentration of 3.75 µg/ml. |
靶标
-
功能Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
-
组织特异性Expressed in liver, colon and small intestine.
-
序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位Nucleus.
- Information by UniProt
-
数据库链接
- Entrez Gene: 8856 Human
- Omim: 603065 Human
- SwissProt: O75469 Human
- Unigene: 7303 Human
-
别名
- BXR antibody
- Nr1i2 antibody
- NR1I2_HUMAN antibody
see all
Anti-PXR antibody 图像
-
Western blot - PXR antibody (ab109728)Anti-PXR antibody (ab109728) at 1 µg/ml + HepG2 cell lysate (RIPA buffer) at 35 µg
Developed using the ECL technique
Predicted band size : 50 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PXR antibody (ab109728)ab109725 at a concentration of 3.75 µg/ml staining PXR in Small Intestine, Formalin-Fixed, Paraffin-Embedded section.
Anti-PXR antibody (ab109728)参考文献
ab109728 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"