概述

  • 产品名称Anti-PXK抗体
    参阅全部 PXK 一抗
  • 描述
    小鼠单克隆抗体to PXK
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Orangutan
  • 免疫原

    Recombinant fragment: PDSVPVDSFP PAPSMAVVAV LESTLSCEAC KNGMPTISRL LQMPLFSDVL LTTSEKPQFK IPTKLKEALR IAKECIEKRL IEEQKQIHQH RRLTRAQSHH, corresponding to amino acids 351-450 of Human PXK (AAH14479) with a proprietory tag.

  • 阳性对照
    • PXK transfected 293T cell lysate. Recombinant tagged human PXK fragment.

性能

应用

Our Abpromise guarantee covers the use of ab77652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 65 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.
  • 组织特异性Widely expressed in all tissues examined except in heart. Isoform 1 is expressed in high levels in the brain, skeletal muscle, spleen and testis. Isoform 7 expression has yet to be demonstrated.
  • 序列相似性Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
    Contains 1 PX (phox homology) domain.
    Contains 1 WH2 domain.
  • 结构域The protein kinase domain is predicted to be catalytically inactive.
  • 细胞定位Cytoplasm. Cell membrane. Also associates with the plasma membrane. Isoform 3 is present throughout the cell.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ20335 antibody
    • K-ATPase antibody
    • Modulator of Na antibody
    • Modulator of Na K ATPase antibody
    • Modulator of Na K ATPase long form antibody
    • MONaKA antibody
    • PX domain containing protein kinase like protein antibody
    • PX domain containing serine/threonine kinase antibody
    • PX domain-containing protein kinase-like protein antibody
    • PX ser/thr kinase v2 antibody
    • PX serine/threonine kinase antibody
    • Pxk antibody
    • PXK_HUMAN antibody
    see all

Anti-PXK antibody 图像

  • Anti-PXK antibody (ab77652) at 1 µg/ml + Recombinant tagged human PXK fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 65 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PXK antibody (ab77652) at 1 µg/ml

    Lane 1 : PXK transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 65 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

Anti-PXK antibody (ab77652)参考文献

ab77652 has not yet been referenced specifically in any publications.

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