概述

  • 产品名称Anti-PWP2H抗体
    参阅全部 PWP2H 一抗
  • 描述
    小鼠多克隆抗体to PWP2H
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    PWP2H (NP_005040.2, 1 a.a. ~ 919 a.a) full-length human protein.

  • 阳性对照
    • HeLa cells, transfected 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 10 µg/ml. Antibody must be purified before use.
    WB: 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-PWP2H antibody 图像

    • All lanes : Anti-PWP2H antibody (ab67848) at 1/500 dilution

      Lane 1 : PWP2H transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 102 kDa
      Observed band size : 102 kDa
    • Immunofluorescence analysis of HeLa cells labelled with purified ab67848 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed using 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.

    Anti-PWP2H antibody (ab67848)参考文献

    This product has been referenced in:
    • Gallenberger M  et al. Lack of WDR36 leads to preimplantation embryonic lethality in mice and delays the formation of small subunit ribosomal RNA in human cells in vitro. Hum Mol Genet 20:422-35 (2011). ICC/IF ; Human . Read more (PubMed: 21051332) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"