PWP2H (NP_005040.2, 1 a.a. ~ 919 a.a) full-length human protein.
HeLa cells, transfected 293T cell lysate
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 10 µg/ml. Antibody must be purified before use.
WB: 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Anti-PWP2H antibody 图像
Western blot - PWP2H antibody (ab67848)
All lanes : Anti-PWP2H antibody (ab67848) at 1/500 dilution Lane 1 : PWP2H transfected 293T cell lysate Lane 2 : Non transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution Developed using the ECL technique Predicted band size : 102 kDa Observed band size : 102 kDa
Immunocytochemistry/ Immunofluorescence - PWP2H antibody (ab67848)
Immunofluorescence analysis of HeLa cells labelled with purified ab67848 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed using 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
Anti-PWP2H antibody (ab67848)参考文献
This product has been referenced in:
et al. Lack of WDR36 leads to preimplantation embryonic lethality in mice and delays the formation of small subunit ribosomal RNA in human cells in vitro. Hum Mol Genet 20:422-35 (2011).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"