概述

  • 产品名称Anti-PUS1抗体
    参阅全部 PUS1 一抗
  • 描述
    兔多克隆抗体to PUS1
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 377 and 427 of human PUS1 (NP_079491.2).

  • 阳性对照
    • Hela whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab85938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.
  • 组织特异性Widely expressed. High levels of expression found in brain and skeletal muscle.
  • 疾病相关Defects in PUS1 are a cause of myopathy with lactic acidosis and sideroblastic anemia type 1 (MLASA1) [MIM:600462]; also known as mitochondrial myopathy and sideroblastic anemia. MLASA is a rare autosomal recessive oxidative phosphorylation disorder specific to skeletal muscle and bone marrow.
  • 序列相似性Belongs to the tRNA pseudouridine synthase TruA family.
  • 细胞定位Mitochondrion and Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A730013B20Rik antibody
    • DOBI antibody
    • MGC112655 antibody
    • MGC11268 antibody
    • MLASA antibody
    • MLASA1 antibody
    • mPus1p antibody
    • Pseudouridine synthase 1 antibody
    • Pseudouridylate synthase 1 antibody
    • PUS1 antibody
    • tRNA pseudouridine synthase A, mitochondrial antibody
    • tRNA pseudouridine(38-40) synthase antibody
    • tRNA pseudouridylate synthase I antibody
    • tRNA uridine isomerase I antibody
    • tRNA-uridine isomerase I antibody
    • TRUA_HUMAN antibody
    see all

Anti-PUS1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PUS1 with ab85938 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-PUS1 antibody (ab85938) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 47 kDa


    Exposure time : 10 seconds
    Note: there are two isoforms of this protein, the second missing amino acids 1-28, which could explain the presence of a double band.
  • Detection of human PUS1 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85938 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 10 seconds.
    Note: there are two isoforms of this protein, the second missing amino acids 1-28, which could explain the presence of a double band.

Anti-PUS1 antibody (ab85938)参考文献

ab85938 has not yet been referenced specifically in any publications.

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