产品名称 Anti-PUS1抗体 参阅全部 PUS1 一抗
WB, IP, IHC-P more details
Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between amino acids 377 and 427 of human PUS1 (NP_079491.2).
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab85938 was affinity purified using an epitope specific to PUS1 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能 Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.
组织特异性 Widely expressed. High levels of expression found in brain and skeletal muscle.
疾病相关 Defects in PUS1 are a cause of myopathy with lactic acidosis and sideroblastic anemia type 1 (MLASA1) [MIM:600462]; also known as mitochondrial myopathy and sideroblastic anemia. MLASA is a rare autosomal recessive oxidative phosphorylation disorder specific to skeletal muscle and bone marrow.
序列相似性 Belongs to the tRNA pseudouridine synthase TruA family.
细胞定位 Mitochondrion and Nucleus.
Information by UniProt
Anti-PUS1 antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS1 antibody (ab85938)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PUS1 with ab85938 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - PUS1 antibody (ab85938)
All lanes : Anti-PUS1 antibody (ab85938) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg developed using the ECL technique Predicted band size : 47 kDa Observed band size : 47 kDa Exposure time : 10 seconds Note: there are two isoforms of this protein, the second missing amino acids 1-28, which could explain the presence of a double band.
Immunoprecipitation - PUS1 antibody (ab85938)
Detection of human PUS1 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85938 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot. Detection: Chemiluminescence with an exposure time of 10 seconds.
Note: there are two isoforms of this protein, the second missing amino acids 1-28, which could explain the presence of a double band.
Anti-PUS1 antibody (ab85938)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"